急性未分化发热性疾病患者白细胞的定量、体积、导电性和散射变化：一项初步研究。
Quantitative and volume, conductivity and scatter changes in leucocytes of patients with acute undifferentiated febrile illness: a pilot study.
作者信息
Kalra Varun, Ahmad Sohaib, Shrivastava Vikas, Mittal Garima
机构信息
Department of Medicine; Pathology; Microbiology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, India-248016.
Department of Medicine; Pathology; Microbiology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, India-248016
出版信息
Trans R Soc Trop Med Hyg. 2016 May;110(5):281-5. doi: 10.1093/trstmh/trw028.
BACKGROUND
A single diagnostic test for acute undifferentiated febrile illnesses (AUFI) is elusive. This pilot study was undertaken on the premise that leucocytes, being the main cells of defence, undergo quantitative, structural and functional changes in AUFI. We evaluated the potential of volume, conductivity and scatter (VCS) parameters of leucocytes, generated with the haemogram report by the Coulter auto-analyzer, in differentiating the common etiologies of AUFI.
METHODS
The haematological and VCS data obtained from 800 controls and 200 cases of AUFI (50 cases each of acute malaria, dengue, scrub typhus and enteric fever) were retrieved for analysis.
RESULTS
The cases and controls differed significantly with respect to relative numbers and the VCS parameters of neutrophils, lymphocytes and monocytes (p<0.05). The neutrophil and lymphocyte were significantly voluminous in acute malaria and scrub typhus as compared to dengue and enteric fevers (p<0.05). Enteric fever significantly enhanced the conductivity of neutrophils as compared to other subgroups while lymphocyte conductivity significantly differed from dengue and scrub typhus. Lymphocyte and neutrophil scatter values in malaria and scrub typhus were comparable but differed significantly from that in enteric fever.
CONCLUSIONS
Etiology-specific changes occur in leucocytes, both in numbers and their VCS properties which can be identified without additional cost.
背景
目前尚未找到针对急性未分化发热性疾病（AUFI）的单一诊断测试方法。本初步研究基于白细胞作为主要防御细胞，在AUFI中会发生数量、结构和功能变化这一前提展开。我们评估了由库尔特自动分析仪生成的血常规报告中白细胞的体积、电导率和散射（VCS）参数在区分AUFI常见病因方面的潜力。
方法
收集了800名对照者和200例AUFI患者（急性疟疾、登革热、恙虫病和伤寒各50例）的血液学和VCS数据进行分析。
结果
病例组和对照组在中性粒细胞、淋巴细胞和单核细胞的相对数量及VCS参数方面存在显著差异（p<0.05）。与登革热和伤寒相比，急性疟疾和恙虫病中的中性粒细胞和淋巴细胞体积显著增大（p<0.05）。与其他亚组相比，伤寒显著提高了中性粒细胞的电导率，而淋巴细胞电导率与登革热和恙虫病有显著差异。疟疾和恙虫病中淋巴细胞和中性粒细胞的散射值相当，但与伤寒中的散射值有显著差异。
结论
白细胞在数量及其VCS特性方面会出现特定病因的变化，且无需额外费用即可识别。
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