Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Pathology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India.
J Clin Lab Anal. 2021 Apr;35(4):e23723. doi: 10.1002/jcla.23723. Epub 2021 Feb 4.
In India, infectious diseases are a leading treatable cause of morbidity and mortality. Mangalore being endemic to many vector-borne diseases, their incidence is known to show seasonal variations with sharp increase during monsoon. Leucocytes have substantial role in the immunological pathogenesis of infections.
The present series was a hospital-based cross-sectional study performed in a tertiary care hospital for a period of three months from June-August wherein the cell population data of cases of malaria, dengue, leptospirosis, typhoid and rickettsial infections along with equal number of healthy controls were collected and analysed. Effectiveness of leucocyte-related volume (V), conductivity (C) and scatter (S) parameters by Coulter®DXH800 haematology analyser in predicting these infections was appraised.
A total of 324 cases comprising of malaria (50%), dengue (30.9%), leptospirosis (13.9%), typhoid (4.0%) and rickettsial infections (1.2%) were included. There was statistically significant differences (P < 0.05) in the mean values of complete blood count parameters-haemoglobin, total leucocyte count, red blood cell count, haematocrit, red cell distribution width, differential leucocyte count, platelet count and plateletcrit between cases and controls and also between specific infections. The mean volumes of neutrophil, monocyte and lymphocyte were considerably increased in malaria and dengue fever compared to leptospirosis, typhoid and rickettsial infections. VCS parameters were the least altered in typhoid fever, except for a strikingly high conductivity and scatter of eosinophils.
Haematological analysis is a part of routine evaluation of any case of febrile illness. This study showed that there are specific alterations in VCS parameters in different types of infections such as malaria, dengue, leptospira, typhoid and rickettsia, the information and analysis of which comes without any additional cost.
在印度，传染病是导致发病率和死亡率的主要可治疗原因。芒格洛尔是许多虫媒传播疾病的流行地，其发病率已知具有季节性变化，在季风期间急剧增加。白细胞在感染的免疫发病机制中起着重要作用。
本系列是一项在三级保健医院进行的基于医院的横断面研究，为期三个月，从 6 月至 8 月，在此期间收集并分析了疟疾、登革热、钩端螺旋体病、伤寒和立克次体感染病例以及相同数量的健康对照者的细胞群体数据。评估 Coulter®DXH800 血液分析仪的白细胞相关体积 (V)、电导率 (C) 和散射 (S) 参数在预测这些感染方面的有效性。
共纳入 324 例病例，包括疟疾（50%）、登革热（30.9%）、钩端螺旋体病（13.9%）、伤寒（4.0%）和立克次体感染（1.2%）。病例组和对照组以及特定感染之间的血红蛋白、总白细胞计数、红细胞计数、血细胞比容、红细胞分布宽度、白细胞分类计数、血小板计数和血小板压积等全血细胞计数参数的平均值存在统计学差异（P<0.05）。与钩端螺旋体病、伤寒和立克次体感染相比，疟疾和登革热患者的中性粒细胞、单核细胞和淋巴细胞体积明显增加。除了嗜酸性粒细胞的电导率和散射率明显升高外，伤寒的 VCS 参数变化最小。
血液分析是任何发热性疾病常规评估的一部分。本研究表明，不同类型的感染（如疟疾、登革热、钩端螺旋体、伤寒和立克次体）存在特定的 VCS 参数变化，这些信息和分析无需额外费用。
