Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
School of Biological Sciences, Building 18, Monash University, Clayton, Victoria 3800, Australia.
Science. 2016 Jul 8;353(6295):aad9969. doi: 10.1126/science.aad9969. Epub 2016 May 19.
Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation-competent myoblasts. However, this model currently lacks in vivo validation. We define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for the asymmetric division of satellite cells driving both self-renewal and regeneration via a clonally restricted progenitor pool.
骨骼肌是一种利用自我更新的干细胞（卫星细胞）来实现再生的组织。最近的体外研究强调了不对称分裂在更新稀有“不朽”干细胞和产生具有分化能力的成肌细胞克隆群体中的作用。然而，该模型目前缺乏体内验证。我们定义了一种类似于哺乳动物卫星细胞的斑马鱼肌肉干细胞群体，并在体内成像了从损伤到纤维替代的整个肌肉再生过程。这项分析揭示了卫星细胞与受损和未受损纤维之间的复杂相互作用，并提供了体内证据，证明卫星细胞的不对称分裂通过克隆限制的祖细胞池来驱动自我更新和再生。
