Salvaggio Antonio, Marino Fabio, Albano Marco, Pecoraro Roberta, Camiolo Giuseppina, Tibullo Daniele, Bramanti Vincenzo, Lombardo Bianca M, Saccone Salvatore, Mazzei Veronica, Brundo Maria V
Experimental Zoo-prophylactic Institute of Sicily Catania, Italy.
Department of Veterinary Science, University of Messina Messina, Italy.
Front Physiol. 2016 Apr 29;7:153. doi: 10.3389/fphys.2016.00153. eCollection 2016.
The increase of heavy metals in the environment involves a high exposure of aquatic organisms to these pollutants. The present study is planned to investigate the effects of zinc chloride (ZnCl2) on the bone embryonic development of Danio rerio and confirm the use of zebrafish as a model organism to study the teratogenic potential of this pollutant. Zebrafish embryos were exposed to different ZnCl2 concentrations and analyzed by ICP-MS. The skeletal anomalies were evaluated to confocal microscope after staining with calcein solution and RhodZin(TM)-3,AM. The data show a delay in hatching compared with the controls, malformations in the process of calcification and significant defects in growth. In conclusion, the current work demonstrates for the first time the Zn toxic effects on calcification process and confirm zebrafish (Danio rerio) as suitable alternative vertebrate model to study the causes and the mechanisms of the skeletal malformations.
环境中重金属含量的增加使水生生物面临着高剂量这些污染物的暴露风险。本研究旨在探究氯化锌（ZnCl₂）对斑马鱼骨骼胚胎发育的影响，并确认斑马鱼作为一种模式生物用于研究该污染物致畸潜力的可行性。将斑马鱼胚胎暴露于不同浓度的ZnCl₂中，并用ICP-MS进行分析。在用钙黄绿素溶液和RhodZin(TM)-3,AM染色后，通过共聚焦显微镜评估骨骼异常情况。数据显示，与对照组相比，孵化延迟，钙化过程中出现畸形，生长存在明显缺陷。总之，当前研究首次证明了锌对钙化过程的毒性作用，并确认斑马鱼（Danio rerio）是研究骨骼畸形原因和机制的合适替代脊椎动物模型。
