Department of Public Health Laboratory Sciences, West China College of Public Health, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
Department of Nutrition and Food Safety, West China College of Public Health, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
Biosci Microbiota Food Health. 2016;35(2):89-96. doi: 10.12938/bmfh.2015-013. Epub 2015 Dec 15.
Fermented milk supplemented with two probiotic strains (Bifidobacterium lactis Bi-07 and Lactobacillus acidophilus NCFM) and a prebiotic (isomaltooligosaccharide) was orally administered to Wistar rats for 30 days using three dosages. A commercial yogurt was used as a placebo. After treatment, the total protein, hemoglobin, and albumin levels in serum were significantly increased in female rats compared with those in the control group (p<0.05), whereas no significant change occurred in the male rats. A significant decrease in serum glucose levels was observed in male rats administered a low dosage of the tested fermented milk (p<0.05). The serum triglyceride level was significantly decreased in both male and female rats (p<0.05). No significant differences were found between rats groups in body weight, food intake, food utilization rate, red blood cell counts, white blood cell counts, alanine aminotransferase, aspartate aminotransferase, urea nitrogen, creatinine, and total cholesterol. These results suggest that the fermented milk supplemented with synbiotics altered the nutritive status of the host animal and contributed to their health. However, such potent health-promoting effects could be deeply associated with the dose and sex specific. Therefore, different physiological targets and population characteristics should be managed with different combinations of probiotics and prebiotics.
发酵乳补充两种益生菌（双歧杆菌 Bi-07 和嗜酸乳杆菌 NCFM）和一种益生元（异麦芽低聚糖），通过三种剂量对 Wistar 大鼠进行口服处理 30 天。商业酸奶用作安慰剂。治疗后，与对照组相比，雌性大鼠血清中的总蛋白、血红蛋白和白蛋白水平显著升高（p<0.05），而雄性大鼠则没有显著变化。接受低剂量测试发酵乳的雄性大鼠的血清葡萄糖水平显著降低（p<0.05）。雄性和雌性大鼠的血清甘油三酯水平均显著降低（p<0.05）。大鼠体重、食物摄入量、食物利用率、红细胞计数、白细胞计数、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、尿素氮、肌酐和总胆固醇在各组之间无显著差异。这些结果表明，补充了合生菌的发酵乳改变了宿主动物的营养状况，并有助于其健康。然而，这种强大的促进健康的作用可能与剂量和性别特异性密切相关。因此，不同的生理目标和人群特征应该通过不同的益生菌和益生元组合来管理。
