Torres-Castro M, Noh-Pech H, Puerto-Hernández R, Reyes-Hernández B, Panti-May A, Hernández-Betancourt S, Yeh-Gorocica A, González-Herrera L, Zavala-Castro J, Puerto F I
Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
PIMVS, Tumbel Kuxtal A.C., Tixkokob, México.
Open Vet J. 2016;6(1):57-61. doi: 10.4314/ovj.v6i1.8. Epub 2016 Mar 28.
Toxoplasma gondii is an obligate intracellular parasite recognized as a causal agent of toxoplasmosis; zoonotic disease endemic in many countries worldwide, including Mexico. Different species of animals participate in the wild cycle infection, including opossums of the species Didelphis virginiana. Thirteen D. virginiana were captured in Yucatan, Mexico. Detection of T. gondii was achieved by Polymerase Chain Reaction, which determined an infection of 76.9% (10/13) in brains. Positive amplicons were sequenced for analysis, this produced results similar to T. gondii with identity and coverage values of 98% and 96-100%, respectively. This study presents the first molecular evidence of the circulation of T. gondii in D. virginiana from Mexico.
刚地弓形虫是一种专性细胞内寄生虫，被认为是弓形虫病的病原体；弓形虫病是一种人畜共患病，在包括墨西哥在内的世界许多国家都有地方流行。不同种类的动物参与野生循环感染，包括弗吉尼亚负鼠（Didelphis virginiana）。在墨西哥尤卡坦捕获了13只弗吉尼亚负鼠。通过聚合酶链反应检测到刚地弓形虫，该方法确定脑部感染率为76.9%（10/13）。对阳性扩增子进行测序分析，结果与刚地弓形虫相似，同一性和覆盖率值分别为98%和96 - 100%。本研究提供了墨西哥弗吉尼亚负鼠中刚地弓形虫传播的首个分子证据。
