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经修饰的核苷三磷酸用于体外选择技术。

Modified Nucleoside Triphosphates for In-vitro Selection Techniques.

机构信息

Laboratorio de Química de Ácidos Nucleicos, INGEBI (CONICET) Ciudad Autónoma de Buenos Aires, Argentina.

Laboratorio de Química de Ácidos Nucleicos, INGEBI (CONICET)Ciudad Autónoma de Buenos Aires, Argentina; Instituto de Ciencias, Universidad Nacional de General SarmientoLos Polvorines, Argentina.

出版信息

Front Chem. 2016 May 4;4:18. doi: 10.3389/fchem.2016.00018. eCollection 2016.

DOI:10.3389/fchem.2016.00018
PMID:27200340
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4854868/
Abstract

The development of SELEX (Selective Enhancement of Ligands by Exponential Enrichment) provides a powerful tool for the search of functional oligonucleotides with the ability to bind ligands with high affinity and selectivity (aptamers) and for the discovery of nucleic acid sequences with diverse enzymatic activities (ribozymes and DNAzymes). This technique has been extensively applied to the selection of natural DNA or RNA molecules but, in order to improve chemical and structural diversity as well as for particular applications where further chemical or biological stability is necessary, the extension of this strategy to modified oligonucleotides is desirable. Taking into account these needs, this review intends to collect the research carried out during the past years, focusing mainly on the use of modified nucleotides in SELEX and the development of mutant enzymes for broadening nucleoside triphosphates acceptance. In addition, comments regarding the synthesis of modified nucleoside triphosphate will be briefly discussed.

摘要

SELEX（指数富集配体系统进化）的发展为寻找具有高亲和力和选择性的配体结合功能寡核苷酸（适体）以及发现具有多种酶活性的核酸序列（核酶和 DNA 酶）提供了一种强大的工具。该技术已广泛应用于天然 DNA 或 RNA 分子的筛选，但为了提高化学和结构多样性，以及在需要进一步化学或生物稳定性的特殊应用中，希望将该策略扩展到修饰的寡核苷酸。考虑到这些需求，本综述旨在收集过去几年的研究成果，主要集中在 SELEX 中修饰核苷酸的使用以及开发突变酶以拓宽核苷三磷酸的接受能力。此外，还将简要讨论修饰核苷三磷酸的合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/6228f1fcd818/fchem-04-00018-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/4d440ae67761/fchem-04-00018-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/59c70f1a7e6a/fchem-04-00018-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/6228f1fcd818/fchem-04-00018-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/4d440ae67761/fchem-04-00018-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/59c70f1a7e6a/fchem-04-00018-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5b4/4854868/6228f1fcd818/fchem-04-00018-g0003.jpg

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