铁-聚（山梨醇-葡萄糖酸）复合物（Ferastral）的测定及其与血清中转铁蛋白的分离
The assay of iron-poly (sorbitol-gluconic acid) complex (Ferastral) and its separation from transferrin in serum.
作者信息
Doney V J, Speyer B E, Fielding J
出版信息
Scand J Haematol Suppl. 1977;32:207-14. doi: 10.1111/j.1600-0609.1977.tb01239.x.
A new iron-poly (sorbitol-gluconic acid) complex (Ferastral) has been studied. A method of assay is described. The iron complex may be separated from serum transferrin using a Sephadex DEAE A50 column. This binds the iron complex and elutes iron-transferrin which can then be assayed. It is shown that the assay of serum transferrin unsaturated binding capacity using excess 59FeCl2 and MgCO3 adsorption, is valid in the presence of Ferastral. Serum unsaturated iron binding capacity may therefore be used to follow the binding of Ferastral iron by transferrin. These methods may be used to follow the distribution of iron in plasma after an intramuscular injection of Ferastral.
一种新型铁-聚（山梨醇-葡萄糖酸）络合物（Ferastral）已被研究。描述了一种测定方法。使用葡聚糖DEAE A50柱可将铁络合物与血清转铁蛋白分离。该柱能结合铁络合物并洗脱铁-转铁蛋白，然后可对其进行测定。结果表明，在存在Ferastral的情况下，使用过量的59FeCl2和MgCO3吸附来测定血清转铁蛋白不饱和结合能力是有效的。因此，血清不饱和铁结合能力可用于追踪转铁蛋白对Ferastral铁的结合情况。这些方法可用于追踪肌肉注射Ferastral后血浆中铁的分布情况。
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