Department of Chemistry, University of Texas, Austin, TX, 78712, USA.
J Am Soc Mass Spectrom. 2016 Sep;27(9):1443-53. doi: 10.1007/s13361-016-1418-9. Epub 2016 May 20.
Dissociation of proteins and peptides by 193 nm ultraviolet photodissociation (UVPD) has gained momentum in proteomic studies because of the diversity of backbone fragments that are produced and subsequent unrivaled sequence coverage obtained by the approach. The pathways that form the basis for the production of particular ion types are not completely understood. In this study, a statistical approach is used to probe hydrogen atom elimination from a + 1 radical ions, and different extents of elimination are found to vary as a function of the identity of the C-terminal residue of the a product ions and the presence or absence of hydrogen bonds to the cleaved residue. Graphical Abstract ᅟ.
蛋白质和肽的解离通过 193nm 紫外光解（UVPD）在蛋白质组学研究中得到了发展，因为该方法产生的肽段种类繁多，随后获得了无与伦比的序列覆盖度。形成特定离子类型基础的途径还不完全清楚。在这项研究中，使用统计方法来探测从+1 自由基离子中消除氢原子，并且发现消除的不同程度随 a 产物离子的 C 末端残基的身份以及与断裂残基的氢键的存在与否而变化。图摘要ᅟ。
