Silva Kássia Oliveira Gomes da, Pereira Sabrina da Conceição, Portovedo Mariana, Milanski Marciane, Galindo Lígia Cristina Monteiro, Guzmán-Quevedo Omar, Manhães-de-Castro Raul, Toscano Ana Elisa
Department of Nutrition, Federal University of Piauí, 64607-670 Picos, Brazil.
Department of Physical Therapy, Federal University of Pernambuco, 50670-901 Recife, Brazil.
Int J Dev Neurosci. 2016 Aug;52:38-45. doi: 10.1016/j.ijdevneu.2016.05.002. Epub 2016 May 19.
Children with cerebral palsy have feeding difficulties that can contribute to undernutrition. The aim of this study was to investigate the effect of early undernutrition on locomotor activity and the expression of the myofibrillar protein MuRF-1 in an experimental model of cerebral palsy (CP). In order to achieve this aim, pregnant rats were divided into two groups according to the diet provided: Normal Protein (NP, n=9) and Low Protein (LP, n=12) groups. After birth, the pups were divided into four groups: Normal Protein Sham (NPS, n=16), Normal Protein Cerebral Palsy (NPCP, n=21), Low Protein Sham (LPS, n=20) and Low Protein Cerebral Palsy (LPCP, n=18) groups. The experimental cerebral palsy protocol consisted of two episodes of anoxia at birth and during the first days of life. Each day, nitrogen flow was used (9l/min during 12min). After nitrogen exposure, sensorimotor restriction was performed 16h per day, from the 2nd to the 28th postnatal day (PND). Locomotor activity was evaluated at 8th, 14th, 17th, 21th and 28th PND. At PND 29, soleus muscles were collected to analyse myofibrillar protein MuRF-1. Our results show that CP animals decreased body weight (p<0.001), which were associated with alterations of various parameters of locomotor activity (p<0.05), compared to their control. Undernourished animals also showed a decrease (p<0.05) in body weight and locomotor activity parameters. Moreover, CP decreased MuRF-1 levels in nourished rats (p=0.015) but not in undernourished rats. In summary, perinatal undernutrition exacerbated the negative effects of cerebral palsy on locomotor activity and muscle atrophy, but it appears not be mediated by changes in MuRF-1 levels.
患有脑瘫的儿童存在进食困难，这可能导致营养不良。本研究的目的是在脑瘫（CP）实验模型中，研究早期营养不良对运动活动及肌原纤维蛋白MuRF-1表达的影响。为实现这一目的，根据所提供的饮食将怀孕大鼠分为两组：正常蛋白（NP，n = 9）组和低蛋白（LP，n = 12）组。出生后，幼崽被分为四组：正常蛋白假手术（NPS，n = 16）组、正常蛋白脑瘫（NPCP，n = 21）组、低蛋白假手术（LPS，n = 20）组和低蛋白脑瘫（LPCP，n = 18）组。实验性脑瘫方案包括出生时及出生后第一天的两次缺氧发作。每天使用氮气流量（12分钟内为9升/分钟）。氮气暴露后，从出生后第2天至第28天，每天进行16小时的感觉运动限制。在出生后第8天、第14天、第17天、第21天和第28天评估运动活动。在出生后第29天，收集比目鱼肌以分析肌原纤维蛋白MuRF-1。我们的结果表明，与对照组相比，CP动物体重下降（p<0.001），这与各种运动活动参数的改变有关（p<0.05）。营养不良的动物体重和运动活动参数也有所下降（p<0.05）。此外，CP降低了营养良好大鼠的MuRF-1水平（p = 0.015），但在营养不良的大鼠中未降低。总之，围产期营养不良加剧了脑瘫对运动活动和肌肉萎缩的负面影响，但似乎不是由MuRF-1水平的变化介导的。
