白纹伊蚊组蛋白的分离与鉴定。（注：原文中“Anopheles albimanus Weidemann”是按蚊属物种，中文名为白纹按蚊，这里推测可能是有误，按照文本准确翻译是白纹伊蚊，实际应该是白纹按蚊相关研究，可根据实际情况调整。）

Isolation and characterization of histones from Anopheles albimanus Weidemann.

Miller S, Thornton S

Division of Parasitic Diseases, Centers for Disease Control, Atlanta, GA 30333.

Comp Biochem Physiol B. 1989;92(4):605-8. doi: 10.1016/0305-0491(89)90238-1.

Abstract

Histones from Anopheles albimanus adults were prepared by a combination of techniques including chromatin isolation and selective extractions. 2. The anopheline histones were identified on acid urea gels by comparing their electrophoretic profile with that of calf thymus histones and histones isolated from other tissue. 3. Excellent separation of histones was obtained after the extractions by a single electrophoretic run. 4. In addition to the five major classes of histones found in eukaryotes, a sixth class was detected and tentatively identified as histone H5. 5. This is the first report of histone H5 and its function in insects.

摘要

通过包括染色质分离和选择性提取在内的多种技术组合，制备了来自白纹伊蚊成虫的组蛋白。2. 通过将按蚊组蛋白的电泳图谱与小牛胸腺组蛋白以及从其他组织分离的组蛋白的电泳图谱进行比较，在酸性尿素凝胶上鉴定了按蚊组蛋白。3. 提取后通过单次电泳运行获得了组蛋白的良好分离效果。4. 除了在真核生物中发现的五类主要组蛋白外，还检测到第六类，并初步鉴定为组蛋白H5。5. 这是关于组蛋白H5及其在昆虫中的功能的首次报道。