从白纹伊蚊（Anopheles albimanus Weidemann）中分离和鉴定染色体蛋白。

Isolation and characterization of chromosomal proteins from the mosquito Anopheles albimanus Weidemann.

Miller S

Division of Parasitic Diseases, Centers for Disease Control, Atlanta, GA 30333.

Int J Biochem. 1988;20(11):1247-53. doi: 10.1016/0020-711x(88)90227-3.

Abstract

Nuclei were isolated from adult anopheline mosquitoes and fractionated into nucleolar chromatin, nucleoplasmic chromatin and ribonucleoprotein particles by sucrose density gradients. 2. Histones and nonhistone proteins were selectively dissociated from chromatin by treatment with sodium chloride, urea and guanidine HC1. 3. A special class of nonhistone proteins (tight proteins) were extracted from chromatin with Na4P2O7. 4. The electrophoretic properties of the histones, nonhistone proteins and ribonucleoprotein particles were examined by isoelectric focusing and SDS multiphase polyacrylamide gel electrophoresis. 5. By contrast to the histones, the nonhistone proteins displayed considerable heterogeneity. 6. Possible functional implications of the chromosomal proteins are discussed.

摘要

从成年按蚊中分离出细胞核，并通过蔗糖密度梯度将其分离为核仁染色质、核质染色质和核糖核蛋白颗粒。2. 通过用氯化钠、尿素和盐酸胍处理，使组蛋白和非组蛋白从染色质中选择性解离。3. 用焦磷酸钠从染色质中提取一类特殊的非组蛋白（紧密蛋白）。4. 通过等电聚焦和十二烷基硫酸钠多相聚丙烯酰胺凝胶电泳检测组蛋白、非组蛋白和核糖核蛋白颗粒的电泳特性。5. 与组蛋白相比，非组蛋白表现出相当大的异质性。6. 讨论了染色体蛋白可能的功能意义。