采用 LC/MS/MS 法同时测定熏肉制品中的 Ltx 和 Ltxd。
Simultaneous determination of Ltx and Ltxd in cured meat products by LC/MS/MS.
机构信息
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China; Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
出版信息
Food Chem. 2016 Nov 1;210:338-43. doi: 10.1016/j.foodchem.2016.04.086. Epub 2016 Apr 19.
9,10-Epoxyoctadec-12-enoic acid (Ltx) and 9,10-dihydroxy-12-octadecenoic acid (Ltxd), oxidation products of Linoleic acid, are of biological significance. In this paper, a method was developed for simultaneous determination of Ltx and Ltxd in cured meat products. The analytes were separated by high performance liquid chromatography and detected with tandem mass spectrometry. The method was of satisfactory performances with the quantification limits of 0.64μg/g and 0.025μg/g for Ltx and Ltxd, respectively. The method was employed to detect the analytes in cured meat products, and 21 out 26 samples were found to contain Ltx and Ltxd simultaneously, in ranges of 0.77-6.90μg/g and 0.17-3.93μg/g, respectively. The result also indicated there were isomers of Ltx and Ltxd in samples, and the final amount of Ltxds after ingestion might be much higher than those originally detected in cured meats. More attention should be paid to Ltxs and Ltxds in cured meat products.
9,10-环氧十八碳-12-烯酸（Ltx）和 9,10-二羟基-12-十八烯酸（Ltxd）是亚油酸的氧化产物，具有生物学意义。本文建立了一种同时测定熟肉制品中 Ltx 和 Ltxd 的方法。采用高效液相色谱-串联质谱法进行检测，两种分析物均能得到很好的分离。Ltx 和 Ltxd 的定量限分别为 0.64μg/g 和 0.025μg/g。该方法用于检测熟肉制品中的分析物，26 个样品中有 21 个样品同时含有 Ltx 和 Ltxd，含量范围分别为 0.77-6.90μg/g 和 0.17-3.93μg/g。结果还表明，样品中存在 Ltx 和 Ltxd 的异构体，摄入后 Ltxds 的总量可能远高于原来在熟肉中检测到的含量。在熟肉制品中应更加关注 Ltxs 和 Ltxds。
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