Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
Stem Cell Rev Rep. 2016 Aug;12(4):454-63. doi: 10.1007/s12015-016-9656-6.
Basal cells (BC) are the stem/progenitor cells of the human airway epithelium capable of differentiating into secretory and ciliated cells. Notch signaling activation increases BC differentiation into secretory cells, but the role of individual Notch ligands in regulating this process in the human airway epithelium is largely unknown. The objective of this study was to define the role of the Notch ligand JAG1 in regulating human BC differentiation. JAG1 over-expression in BC increased secretory cell differentiation, with no effect on ciliated cell differentiation. Conversely, knockdown of JAG1 decreased expression of secretory cell genes. These data demonstrate JAG1-mediated Notch signaling regulates differentiation of BC into secretory cells.
基底细胞（BC）是人类气道上皮的干细胞/祖细胞，能够分化为分泌细胞和纤毛细胞。Notch 信号通路的激活可增加 BC 向分泌细胞分化，但 Notch 配体在调节人类气道上皮细胞这一过程中的作用在很大程度上是未知的。本研究的目的是确定 Notch 配体 JAG1 在调节人类 BC 分化中的作用。BC 中 JAG1 的过表达增加了分泌细胞的分化，而对纤毛细胞分化没有影响。相反，JAG1 的敲低降低了分泌细胞基因的表达。这些数据表明 JAG1 介导的 Notch 信号通路调节 BC 向分泌细胞的分化。
