Aoyama Y, Chan Y L, Wool I G
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
FEBS Lett. 1989 May 22;249(1):119-22. doi: 10.1016/0014-5793(89)80028-6.
The amino acid sequence of rat ribosomal protein L34 was deduced from the nucleotide sequence in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Ribosomal protein L34 contains 117 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has an Mr of 13,498. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 7-9 copies of the L34 gene. The mRNA for the protein is about 630 nucleotides in length.
大鼠核糖体蛋白L34的氨基酸序列是从重组cDNA的核苷酸序列推导出来的,并通过该蛋白的氨基末端氨基酸序列得到证实。核糖体蛋白L34含有117个氨基酸(mRNA翻译后氨基末端的甲硫氨酸被去除),分子量为13498。cDNA与核DNA消化产物的杂交表明,L34基因有7-9个拷贝。该蛋白的mRNA长度约为630个核苷酸。