大鼠核糖体蛋白S10的一级结构。

The primary structure of rat ribosomal protein S10.

作者信息

Glück A, Chan Y L, Lin A, Wool I G

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.

出版信息

Eur J Biochem. 1989 Jun 1;182(1):105-9. doi: 10.1111/j.1432-1033.1989.tb14805.x.

DOI:10.1111/j.1432-1033.1989.tb14805.x

PMID:2543570

Abstract

The amino acid sequence of rat ribosomal protein S10 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Ribosomal protein S10 contains 165 amino acids and has a molecular mass of 18917 Da. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 17-20 copies of the S10 gene. The mRNA for the protein is about 750 nucleotides in length. Ribosomal protein S10 has several possible internal duplications; one is a tandem repeat of ten residues that is basic and contains two or three prolines.

摘要

大鼠核糖体蛋白S10的氨基酸序列是从重组cDNA的核苷酸序列推导出来的，并通过该蛋白的NH2末端氨基酸序列得到证实。核糖体蛋白S10含有165个氨基酸，分子量为18917道尔顿。cDNA与核DNA消化产物的杂交表明，S10基因有17 - 20个拷贝。该蛋白的mRNA长度约为750个核苷酸。核糖体蛋白S10有几个可能的内部重复序列；其中一个是十个残基的串联重复序列，该序列呈碱性，含有两到三个脯氨酸。