Chang Shuai, He Shicai, Qiu Guanglin, Lu Jing, Wang Jin, Liu Junsong, Fan Lin, Zhao Wei, Che Xiangming
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi Province, 710061, China.
Tumour Biol. 2016 Sep;37(9):12141-12151. doi: 10.1007/s13277-016-5094-y. Epub 2016 May 24.
MicroRNAs have been documented playing key roles in cancer development and progression. Here, we investigate the role of miR-125b in gastric cancer metastasis. We found that the expression of miR-125b was up-regulated in gastric cancer tissue specimens compared with their corresponding nontumorous tissues, and the up-regulated miR-125b level was significantly associated with TNM stage and lymph node-metastasis. Overexpression of miR-125b promoted gastric cancer cell migration and invasion in vitro and metastasis in vivo. STARD13 and NEU1 were identified as direct target genes of miR-125b by luciferase assays, and they were involved in the cell migration and invasion regulated by miR-125b in gastric cancer. Taken together, miR-125b functions as an oncogene in gastric cancer and represents a new potential therapeutic target for gastric cancer.
微小RNA已被证明在癌症发生和发展中发挥关键作用。在此，我们研究了miR-125b在胃癌转移中的作用。我们发现，与相应的非肿瘤组织相比，miR-125b在胃癌组织标本中的表达上调，且上调的miR-125b水平与TNM分期和淋巴结转移显著相关。miR-125b的过表达促进了胃癌细胞的体外迁移和侵袭以及体内转移。通过荧光素酶测定法确定STARD13和NEU1为miR-125b的直接靶基因，它们参与了miR-125b在胃癌中调节的细胞迁移和侵袭。综上所述，miR-125b在胃癌中作为癌基因发挥作用，是胃癌新的潜在治疗靶点。
