Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Egypt.
Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
Asian Pac J Cancer Prev. 2022 Nov 1;23(11):3771-3777. doi: 10.31557/APJCP.2022.23.11.3771.
Micro-RNAs (miRNAs) are post-transcriptional regulators of gene expression that are abundantly expressed in a variety of cancers, including breast cancer. The mechanism of miRNAs in breast cancer oncogenesis is poorly understood. The goal of this study was to determine if there was a link between the miR-423 rs6505162 gene variation and breast cancer susceptibility among Egyptian patients.
This was a case control study that included 120 female patients with pathologically confirmed breast cancer and 120 healthy controls. The patients and controls were genotyped for miR-423 rs6505162 polymorphism by real time PCR. The association of breast cancer patients' genotypic variant and clinicopathological characteristics was analyzed.
Breast cancer patients showed significantly higher AA and CA genotypes frequencies when compared to controls. This was translated as higher risk to develop breast cancer in patients harboring these genotypic variants (OR = 3.28, p= 0.002; OR = 2.11, p= 0.011, respectively). The frequencies of Her2 positive and advanced stage disease were significantly increased in the AA genotype variant (p<0.001).
Our data suggest that miR-423 rs6505162 polymorphism could be a potential risk factor in the pathogenesis of breast cancer among Egyptian population.
Micro-RNAs（miRNAs）是基因表达的转录后调控因子，在多种癌症中大量表达，包括乳腺癌。miRNAs 在乳腺癌发生中的作用机制尚不清楚。本研究旨在确定 miR-423 rs6505162 基因变异与埃及患者乳腺癌易感性之间是否存在关联。
这是一项病例对照研究，包括 120 名经病理证实的乳腺癌女性患者和 120 名健康对照。通过实时 PCR 对 miR-423 rs6505162 多态性进行基因分型。分析了乳腺癌患者基因型变异与临床病理特征的相关性。
与对照组相比，乳腺癌患者的 AA 和 CA 基因型频率明显升高。这表明携带这些基因型变异的患者发生乳腺癌的风险更高（OR=3.28，p=0.002；OR=2.11，p=0.011）。AA 基因型变异患者中 Her2 阳性和晚期疾病的频率显著增加（p<0.001）。
我们的数据表明，miR-423 rs6505162 多态性可能是埃及人群乳腺癌发病机制中的一个潜在危险因素。
