He L B, Wang H, Luo L F, Jiang S H, Liu L Y, Li Y M, Huang R, Liao L J, Zhu Z Y, Wang Y P
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
J Fish Biol. 2016 Aug;89(2):1434-40. doi: 10.1111/jfb.12997. Epub 2016 May 25.
In this study, the toll-like receptor 1 (tlr1) and toll-like receptor 2 (tlr2) genes of grass carp Ctenopharyngodon idella were cloned and characterized. tlr1 and tlr2 were found to be highly expressed in immune system organs such as spleen, middle kidney and heart kidney. The expression level of tlr1 and tlr2 was found to be up-regulated at the later stage of viral challenge process. Moreover, subcellular localization indicated that Tlr1 and Tlr2 shared similar localization pattern and both of them may locate in the plasma membrane of transfected cells.
在本研究中，克隆并鉴定了草鱼（Ctenopharyngodon idella）的Toll样受体1（tlr1）和Toll样受体2（tlr2）基因。发现tlr1和tlr2在脾脏、中肾和心脏肾脏等免疫系统器官中高度表达。在病毒攻击过程后期，tlr1和tlr2的表达水平被发现上调。此外，亚细胞定位表明，Tlr1和Tlr2具有相似的定位模式，它们都可能定位于转染细胞的质膜上。
