Margheri Giancarlo, Zoppi Angela, Olmi Roberto, Trigari Silvana, Traversi Rita, Severi Mirko, Bani Daniele, Bianchini Francesca, Torre Eugenio, Margheri Francesca, Chillà Anastasia, Biagioni Alessio, Calorini Lido, Laurenzana Anna, Fibbi Gabriella, Del Rosso Mario
Institute for Complex Systems, National Research Council, Sesto Fiorentino, Italy.
Department of Physics "Enrico Fermi", University of Pisa, Italy.
Oncotarget. 2016 Jun 28;7(26):39846-39860. doi: 10.18632/oncotarget.9511.
In the photothermal treatments (PTs) of tumor, the localization of a high number of near-infrared (NIR) absorbing gold nanoparticles in the tumor mass is still a challenging issue. Here, we propose a promising strategy to deliver therapeutic chitosan-coated gold nanoparticles to tumor cells as hidden cargo of Endothelial Colony Forming Cells (ECFCs) endowed with an innate tumor-tropism. Remarkably, ECFC gold enrichement doesn't affect cell viability and preserves the endothelial lineage characteristics such as capillary morphogenesis and cell migration. We demonstrate that heavily Au-doped ECFCs are able to efficiently warm up the tumor environment, and kill the cancer cells via hyperthermic heating both in vitro as well as in vivo. Thus, we show an excellent thermotransductive property of gold enriched ECFCs and their capability to kill melanoma cells at moderate NIR light intensities.
在肿瘤的光热治疗（PTs）中，使大量近红外（NIR）吸收性金纳米颗粒定位在肿瘤块中仍然是一个具有挑战性的问题。在此，我们提出了一种有前景的策略，即将治疗性壳聚糖包被的金纳米颗粒作为具有天然肿瘤嗜性的内皮祖细胞（ECFCs）的隐藏 cargo 递送至肿瘤细胞。值得注意的是，ECFC 金富集不影响细胞活力，并保留了内皮谱系特征，如毛细血管形态发生和细胞迁移。我们证明，大量掺金的 ECFCs 能够有效加热肿瘤环境，并在体外和体内通过热疗加热杀死癌细胞。因此，我们展示了富含金的 ECFCs 的优异热传导特性及其在中等 NIR 光强度下杀死黑色素瘤细胞的能力。
