热带假丝酵母和白色念珠菌腺嘌呤营养缺陷型原生质体融合的种间互补分析。
Interspecific complementation analysis by protoplast fusion of Candida tropicalis and Candida albicans adenine auxotrophs.
作者信息
Corner B E, Poulter R T
机构信息
Biochemistry Department, University of Otago, Dunedin, New Zealand.
出版信息
J Bacteriol. 1989 Jun;171(6):3586-9. doi: 10.1128/jb.171.6.3586-3589.1989.
Abstract
A protocol employing inositol starvation was used to isolate proline and adenine auxotrophs of Candida tropicalis. Interspecific hybrids between red adenine auxotrophs of C. tropicalis and Candida albicans were formed by protoplast fusion. These C. tropicalis red adenine auxotrophs were shown to fall into two complementation groups by crossing them with a known C. albicans ade1 tester strain. It is suggested that these two groups correspond to the ade1 and ade2 mutants of Saccharomyces cerevisiae and C. albicans and that these defined mutants may be useful in attempts to develop transformation systems for C. tropicalis.
摘要
采用肌醇饥饿法分离热带假丝酵母的脯氨酸和腺嘌呤营养缺陷型。通过原生质体融合，在热带假丝酵母的红色腺嘌呤营养缺陷型与白色念珠菌之间形成了种间杂种。通过将这些热带假丝酵母红色腺嘌呤营养缺陷型与已知的白色念珠菌ade1测试菌株杂交，表明它们可分为两个互补群。有人提出，这两个互补群分别对应于酿酒酵母和白色念珠菌的ade1和ade2突变体，并且这些确定的突变体可能有助于开发热带假丝酵母的转化系统。
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