用于选择粗糙脉孢菌营养缺陷型突变体的高效方法。
Efficient method for selection of auxotrophic mutants of Neurospora.
作者信息
LESTER H E, GROSS S R
出版信息
Science. 1959 Feb 27;129(3348):572. doi: 10.1126/science.129.3348.572.
PMID:13634996
Abstract
A technique for the selection of induced and spontaneous auxotrophic mutants of Neurospora in relatively high frequency is described. The method takes advantage of the reduced rate of death of germinating conidia of an inositol-requiring strain when a secondary mutation is imposed.
摘要
本文描述了一种以相对高频率筛选粗糙脉孢菌诱导型和自发营养缺陷型突变体的技术。该方法利用了当施加二次突变时，需肌醇菌株的萌发分生孢子死亡率降低这一特性。
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