通过原生质体融合对白色念珠菌进行准性遗传分析。
Parasexual genetic analysis of Candida albicans by spheroplast fusion.
作者信息
Poulter R, Jeffery K, Hubbard M J, Shepherd M G, Sullivan P A
出版信息
J Bacteriol. 1981 Jun;146(3):833-40. doi: 10.1128/jb.146.3.833-840.1981.
Abstract
Doubly auxotrophic strains of Candida albicans were selected from mutagenized cultures. Spheroplasts prepared from the auxotrophic strains were fused with polyethylene glycol. Prototrophic derivatives formed by this fusion protocol from auxotrophic strains were selected by complementation on minimal medium. These prototrophs had a cell volume twice that of the original strain and were shown to be heterozygous at four loci. Prototrophs obtained by this procedure infrequently gave rise to auxotrophic recombinants whose cell volume remained twice that of the original strain. It is suggested that these auxotrophic recombinants arise from mitotic crossing-over. This paper is the first report of a parasexual cycle in C. albicans.
摘要
从诱变培养物中筛选出白色念珠菌的双营养缺陷型菌株。从营养缺陷型菌株制备的原生质体用聚乙二醇进行融合。通过这种融合方案从营养缺陷型菌株形成的原养型衍生物通过在基本培养基上的互补作用进行筛选。这些原养型菌株的细胞体积是原始菌株的两倍，并且在四个位点上表现为杂合。通过该程序获得的原养型菌株很少产生细胞体积仍为原始菌株两倍的营养缺陷型重组体。有人认为这些营养缺陷型重组体是由有丝分裂交换产生的。本文是关于白色念珠菌准性周期的首次报道。
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