Kang Tae-Yun, Hong Jung Min, Jung Jin Woo, Kang Hyun-Wook, Cho Dong-Woo
Department of Biomedical Engineering, Yale University, New Haven, Connecticut, United States of America.
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, Korea.
PLoS One. 2016 May 26;11(5):e0156529. doi: 10.1371/journal.pone.0156529. eCollection 2016.
We used indirect stereolithography (SL) to form inner-layered fluidic networks in a porous scaffold by introducing a hydrogel barrier on the luminal surface, then seeded the networks separately with human umbilical vein endothelial cells and human lung fibroblasts to form a tissue mimic containing vascular networks. The artificial vascular networks provided channels for oxygen transport, thus reducing the hypoxic volume and preventing cell death. The endothelium of the vascular networks significantly retarded the occlusion of channels during whole-blood circulation. The tissue mimics have the potential to be used as an in vitro platform to examine the physiologic and pathologic phenomena through vascular architecture.
我们采用间接立体光刻技术（SL），通过在管腔表面引入水凝胶屏障，在多孔支架中形成内层流体网络，然后分别将人脐静脉内皮细胞和人肺成纤维细胞接种到这些网络中，以形成包含血管网络的组织模拟物。人工血管网络提供了氧气运输通道，从而减少了缺氧体积并防止细胞死亡。血管网络的内皮在全血循环过程中显著延缓了通道的闭塞。这些组织模拟物有潜力用作体外平台，通过血管结构来研究生理和病理现象。
