Miranda Alice, Pêgo José M, Correia-Pinto Jorge
1 Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.
2 ICVS/3Bs - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Lab Anim. 2017 Apr;51(2):204-207. doi: 10.1177/0023677216652342. Epub 2016 Jul 9.
Endotracheal intubation of laboratory animals is a common procedure shared by several research fields for different purposes, such as mechanical ventilation of anaesthetized animals, instillation of cytotoxic nanoparticles, infectious agents or tumour cells for induction of disease models, and even for diagnostic and therapeutic purposes. These different research purposes, achieved in different animal models, require technical expertise and equipment that suits every research need from animal facilities. In this short report we propose a videoendoscopic intubation station that could be shared among the most common laboratory animals, namely the mouse, rat, guinea pig and rabbit, from neonates to adult animals. This report aims to contribute to the reduction of animals excluded from experiments due to false paths during direct and blind intubations and to the refinement of procedures by replacing surgical approaches such as tracheotomy.
对实验动物进行气管插管是多个研究领域出于不同目的而常用的操作,比如对麻醉动物进行机械通气、滴注细胞毒性纳米颗粒、感染因子或肿瘤细胞以诱导疾病模型,甚至用于诊断和治疗目的。在不同动物模型中实现的这些不同研究目的,需要适合动物设施各种研究需求的技术专长和设备。在本简短报告中,我们提出了一种视频内镜插管工作站,它可在最常见的实验动物(即从新生动物到成年动物的小鼠、大鼠、豚鼠和兔子)之间共享。本报告旨在减少因直接插管和盲目插管过程中的错误路径而被排除在实验之外的动物数量,并通过取代诸如气管切开术等外科手术方法来完善操作程序。