Univ-Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, Département de Pharmacologie, CIC 1401, F-33000, Bordeaux, France.
INSERM, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, CIC 1401, F-33000, Bordeaux, France.
Respir Res. 2019 Sep 14;20(1):211. doi: 10.1186/s12931-019-1177-9.
Animal models and, in particular, mice models, are important tools to investigate the pathogenesis of respiratory diseases and to test potential new therapeutic drugs. Lung function measurement is a key step in such investigation. In mice, it is usually performed using forced oscillation technique (FOT), negative pressure-driven forced expiratory (NPFE) and pressure-volume (PV) curve maneuvers. However, these techniques require a tracheostomy, which therefore only allows end-point measurements. Orotracheal intubation has been reported to be feasible and to give reproducible lung function measurements, but the agreement between intubation and tracheostomy generated-data remains to be tested.
Using the Flexivent system, we measured lung function parameters (in particular, forced vital capacity (FVC), forced expiratory volume in the first 0.1 s (FEV0.1), compliance (Crs) of the respiratory system, compliance (C) measured using PV loop and an estimate of inspiratory capacity (A)) in healthy intubated BALB/cJ mice and C57BL/6 J mice and compared the results with similar measurements performed in the same mice subsequently tracheostomized after intubation, by means of paired comparison method, correlation and Bland-Altman analysis. The feasibility of repetitive lung function measurements by intubation was also tested.
We identified parameters that are accurately evaluated in intubated animals (i.e., FVC, FEV0.1, Crs, C and A in BALB/cJ and FVC, FEV0.1, and A in C57BL/6 J). Repetitive lung function measurements were obtained in C57BL/6 J mice.
This subset of lung function parameters in orotracheally intubated mice is reliable, thereby allowing relevant longitudinal studies.
动物模型，特别是小鼠模型，是研究呼吸道疾病发病机制和测试潜在新治疗药物的重要工具。肺功能测量是此类研究的关键步骤。在小鼠中，通常使用强迫振荡技术（FOT）、负压驱动呼气（NPFE）和压力-容积（PV）曲线操作来进行。然而，这些技术需要进行气管切开术，因此只能进行终点测量。已经有报道称经口气管插管是可行的，并可获得可重复的肺功能测量结果，但插管和气管切开术生成数据之间的一致性仍有待检验。
我们使用 Flexivent 系统测量了健康插管的 BALB/cJ 小鼠和 C57BL/6J 小鼠的肺功能参数（特别是用力肺活量（FVC）、第 0.1 秒用力呼气量（FEV0.1）、呼吸系统顺应性（Crs）、使用 PV 环测量的顺应性（C）和吸气量估计值（A）），并通过配对比较法、相关性和 Bland-Altman 分析比较了这些结果与随后在同一批小鼠中进行气管切开术的类似测量结果。我们还测试了通过插管进行重复肺功能测量的可行性。
我们确定了在插管动物中可准确评估的参数（即 BALB/cJ 中的 FVC、FEV0.1、Crs、C 和 A，以及 C57BL/6J 中的 FVC、FEV0.1 和 A）。在 C57BL/6J 小鼠中获得了重复的肺功能测量结果。
经口气管插管小鼠的这组肺功能参数可靠，从而允许进行相关的纵向研究。
