利用双折射变化对神经动作电位进行实时成像。
Real-time imaging of action potentials in nerves using changes in birefringence.
作者信息
Badreddine Ali H, Jordan Tomas, Bigio Irving J
机构信息
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Department of Electrical & Computer Engineering, Boston University, Boston, MA 02215, USA; Boston University Photonics Center, Boston University, Boston, MA 02215, USA.
出版信息
Biomed Opt Express. 2016 Apr 21;7(5):1966-73. doi: 10.1364/BOE.7.001966. eCollection 2016 May 1.
Polarized light can be used to measure the electrical activity associated with action potential propagation in nerves, as manifested in simultaneous dynamic changes in their intrinsic optical birefringence. These signals may serve as a tool for minimally invasive neuroimaging in various types of neuroscience research, including the study of neuronal activation patterns with high spatiotemporal resolution. A fast linear photodiode array was used to image propagating action potentials in an excised portion of the lobster walking leg nerve. We show that the crossed-polarized signal (XPS) can be reliably imaged over a ≥2 cm span in our custom nerve chamber, by averaging multiple-stimulation signals, and also in single-scan real-time "movies". This demonstration paves the way toward utilizing changes in the optical birefringence to image more complex neuronal activity in nerve fibers and other organized neuronal tissue.
偏振光可用于测量与神经中动作电位传播相关的电活动，这表现为其固有光学双折射的同步动态变化。这些信号可作为一种工具，用于各类神经科学研究中的微创神经成像，包括对具有高时空分辨率的神经元激活模式的研究。使用快速线性光电二极管阵列对龙虾步足神经的离体部分中传播的动作电位进行成像。我们表明，通过对多个刺激信号进行平均，以及在单次扫描实时“电影”中，交叉偏振信号（XPS）能够在我们定制的神经腔室中≥2厘米的跨度上可靠成像。这一演示为利用光学双折射的变化来成像神经纤维和其他有组织的神经元组织中更复杂的神经元活动铺平了道路。
Zotero 插件