Luc Djogbénou S, Benoit Assogba, Laurette Djossou, Michel Makoutode
Institut Régional de Santé Publique, Université d'Abomey Calavi, 01BP918 Cotonou, Bénin.
J Vector Ecol. 2016 Jun;41(1):34-40. doi: 10.1111/jvec.12191.
We investigated the possible relationship between the agricultural use of insecticides and the emergence of insecticide resistance. Bioassays were conducted using simulated mosquito larval habitats and well known Anopheles gambiae strains. Soil samples were collected from vegetable production areas in Benin, including one site with insecticide use, one site where insecticides had not been used for two months, and a third where insecticides had not been used. Pupation and emergence rates were very low in pyrethroid-susceptible strains when exposed to soil that had been recently exposed to insecticides. Pupation and emergence rates in strains with the kdr mutation alone or both the kdr and Ace-1 mutations were much higher. Overall, strains with the kdr mutation survived at higher rates compared to that without kdr mutation. Although this study is observational, we provide indirect evidence indicating that soils from agricultural areas contain insecticide residues that can play a role in the emergence of insecticide resistance in Anopheles. This aspect should be taken into account to better utilize the insecticide in the context of integrated pest management programs.
我们调查了杀虫剂的农业使用与杀虫剂抗性出现之间的可能关系。使用模拟蚊虫幼虫栖息地和著名的冈比亚按蚊品系进行了生物测定。从贝宁的蔬菜生产区采集土壤样本，包括一个使用杀虫剂的地点、一个两个月未使用杀虫剂的地点以及第三个从未使用过杀虫剂的地点。当暴露于最近接触过杀虫剂的土壤时，拟除虫菊酯敏感品系的化蛹率和羽化率非常低。仅具有kdr突变或同时具有kdr和Ace-1突变的品系的化蛹率和羽化率要高得多。总体而言，具有kdr突变的品系比没有kdr突变的品系存活率更高。尽管这项研究是观察性的，但我们提供了间接证据表明，农业地区的土壤含有杀虫剂残留，这些残留可能在冈比亚按蚊杀虫剂抗性的出现中发挥作用。在综合虫害管理计划的背景下，为了更好地使用杀虫剂，应考虑这一方面。
