Wei L, Shan Z G, Flower R L, Wang Z, Wen J Z, Luo G P, Ji Y L
Institute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou, China.
Clinical Services and Research, Australian Red Cross Blood Service, Brisbane, Australia.
Vox Sang. 2016 Oct;111(3):308-314. doi: 10.1111/vox.12421. Epub 2016 May 27.
MNS hybrid glycophorins are identified by characteristic antigen profiles. One of these is the Mur antigen, which is expressed on red cell hybrid glycophorins of several phenotypes of the 'Miltenberger' series found predominantly in East Asian population. The aim of this study was to investigate the distribution of Mur-positive hybrid glycophorins and clarify the genetic basis in the donors from southern China.
Blood samples from 528 donors were collected for Mur antigen serological typing. Sequencing of GYPB pseudoexon 3 and MNS phenotyping were conducted in Mur-positive samples. The multiplex ligation-dependent probe amplification (MLPA) was used to confirm the zygosity of the GYP.Mur allele and determine the MNSs genotype. The expression of Mur antigen was evaluated by flow cytometry.
Fifty-one Mur-positive samples were identified by serological testing. Sequencing analysis showed 50 donors (50/528, 9.5%) with the GYP.Mur allele (48 heterozygotes and two homozygotes), which were confirmed by the MLPA genotyping analysis, and one donor (1/528, 0.19%) with a novel GYP.Bun allele. Flow cytometry analysis revealed higher Mur antigen expression on GP.Mur (Mi.III) homozygotes than heterozygotes. For the GYP.Mur homozygotes, an incorrect 'N' positive typing with anti-N lectin was obtained.
GP.Mur (Mi.III) is the main Mur-positive hybrid glycophorin in Guangzhou donors. The dosage effect of Mur antigen observed provides a basis for selecting the homozygous GP.Mur RBCs as the reagent cells to avoid neglecting weak antibodies. A separate GYP.Bun lineage found in the southern China provides evidence for further complexity in the MNS system.
MNS杂合血型糖蛋白通过特征性抗原谱得以鉴定。其中之一是Mur抗原，它表达于主要在东亚人群中发现的“米尔滕贝格”系列几种表型的红细胞杂合血型糖蛋白上。本研究的目的是调查Mur阳性杂合血型糖蛋白的分布情况，并阐明中国南方献血者的遗传基础。
采集528名献血者的血样进行Mur抗原血清学分型。对Mur阳性样本进行GYPB假外显子3测序和MNS表型分析。采用多重连接依赖探针扩增（MLPA）技术确认GYP.Mur等位基因的纯合性并确定MNSs基因型。通过流式细胞术评估Mur抗原的表达。
通过血清学检测鉴定出51份Mur阳性样本。测序分析显示50名献血者（50/528，9.5%）携带GYP.Mur等位基因（48名杂合子和2名纯合子），经MLPA基因分型分析得以证实，还有1名献血者（1/528，0.19%）携带新型GYP.Bun等位基因。流式细胞术分析显示，GP.Mur（Mi.III）纯合子上的Mur抗原表达高于杂合子。对于GYP.Mur纯合子，使用抗N凝集素获得了错误的“N”阳性分型结果。
GP.Mur（Mi.III）是广州献血者中主要的Mur阳性杂合血型糖蛋白。观察到的Mur抗原剂量效应为选择纯合GP.Mur红细胞作为试剂细胞以避免忽视弱抗体提供了依据。在中国南方发现的一个单独的GYP.Bun谱系为MNS系统的进一步复杂性提供了证据。
