Immucor, Warren, New Jersey.
Immucor, Norcross, Georgia.
Transfusion. 2019 Dec;59(12):3767-3775. doi: 10.1111/trf.15584. Epub 2019 Nov 7.
The GP.Mur glycophorin with Mi phenotype is relatively common and clinically significant in the Southeast Asian populations. The aim of this study is to genotype Mi -positive Asian American type O blood donors. Red blood cell (RBC) minor antigens were also determined in the same cohort.
Asian American blood donors of the Gulf Coast Regional Blood Center (Houston, TX) were screened using a typing reagent (NOVACLONE Anti-Mi Monoclonal IgG Typing Reagent, Dominion Biologicals Ltd) from March 2016 to July 2018. Aliquots of Mi -positive blood from type O donors were subjected to serologic confirmation using Mi - and/or Mur-specific GAMA210 and 64D6 monoclonal antibodies, and two human antisera. Extracted genomic DNA was amplified by polymerase chain reaction (PCR) using GYP hybrid gene/allele-specific primers followed by bidirectional Sanger sequencing. Zygosity for GYPMur and GYPBun was determined using TaqMan real-time PCR assay. Phenotypes of 35 RBC antigens and three phenotypic variants were determined with use of an in vitro diagnostic test, PreciseType HEA Molecular BeadChip Test (Immucor).
By screening 4600 blood donations in the Houston metropolitan area, 209 samples from 103 unique donors were identified to be Mi -positive. By PCR and sequencing analysis, 97 of the 103 Mi -positive donors carried hybrid genes GYPMur (89.7% including two homozygotes), GYPBun (6.2%), GYPVw (3.1%) and GYPHut (1.0%). Concordance between serology and DNA analysis was 98%, 99%, and 100% for the GAMA210, 64D6, and human antisera, respectively. Genotyping of RBC antigens showed that the Mi -positive donors were predominantly associated M+ N- S- s+ (48.5%) and M+ N+ S- s+ (38.1%) phenotypes.
The GP.Mur glycophorin is most prevalent in the Mi -positive Asian American type O blood donors.
在东南亚人群中，具有 Mi 表型的 GP.Mur 血型糖蛋白较为常见且具有重要的临床意义。本研究旨在对 Mi 阳性的亚裔美国 O 型血供者进行基因分型。在同一队列中还确定了红细胞（RBC）的次要抗原。
2016 年 3 月至 2018 年 7 月，使用 NOVACLONE Anti-Mi 单克隆 IgG 定型试剂（Dominion Biologicals Ltd）对来自海湾海岸地区血液中心（休斯顿，德克萨斯州）的亚裔美国献血者进行筛查。从 Mi 阳性 O 型供者的血样中提取的 Mi 阳性血样用 Mi 和/或 Mur 特异性 GAMA210 和 64D6 单克隆抗体以及两种人抗血清进行血清学确认。使用 GYP 杂种基因/等位基因特异性引物通过聚合酶链反应（PCR）扩增提取的基因组 DNA，然后进行双向 Sanger 测序。使用 TaqMan 实时 PCR 检测法确定 GYPMur 和 GYPBun 的杂合性。使用体外诊断测试 PreciseType HEA Molecular BeadChip Test（Immucor）测定 35 种 RBC 抗原和 3 种表型变异的表型。
在休斯顿大都市区筛查 4600 份献血样本后，从 103 位独特供者中确定了 209 份 Mi 阳性样本。通过 PCR 和测序分析，在 103 位 Mi 阳性供者中，97 位携带杂交基因 GYPMur（包括 2 位纯合子在内的 89.7%）、GYPBun（6.2%）、GYPVw（3.1%）和 GYPHut（1.0%）。GAMA210、64D6 和人抗血清的血清学和 DNA 分析之间的一致性分别为 98%、99%和 100%。对 RBC 抗原进行基因分型显示，Mi 阳性供者主要与 M+N-S-s+（48.5%）和 M+N+S-s+（38.1%）表型相关。
GP.Mur 血型糖蛋白在 Mi 阳性的亚裔美国 O 型血供者中最为常见。
