Yang Meng-Hua, Chen Jen-Wei, Sayaka Kaito, Uchikawa Makoto, Tsuno Nelson H, Wei Sheng-Tang, Hou Sheng-Mou, Chen Yann-Jang
Head Office, Taiwan Blood Services Foundation, Taipei 10066, Taiwan.
Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan.
Diagnostics (Basel). 2021 Apr 29;11(5):806. doi: 10.3390/diagnostics11050806.
Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mi, anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mi (377T) binds to DXHKRDTYA, HKRDTYAAHT peptides, and anti-Mi (367T) binds to QTNDXHKRD peptides (X indicates T, M, or K). Anti-Mur is reactive with KRDTYPAHTA peptides. Anti-MUT is reactive with KHKRDTYA. A total of 78,327 donors were screened using three MoAbs, and 3690 (4.71%) were GP.Mur, 20 (0.025%) were GP.Hut, and 18 (0.022%) were GP.Vw. When the Mi antigen was introduced as routine screening, the frequency of Mi(a+) among blood donors in Taiwan was 4.66% (67,348/1,444,541). Mi antigen was implemented as a routine blood testing, and the results were labeled on all red blood cell (RBC) units.
诸如GP.Mur之类的血型糖蛋白杂合体在东南亚人群中很常见。在台湾，针对GP.Mur血型表型具有临床意义的同种抗体是血库中最重要的问题。迫切需要对台湾人群进行大规模的血型糖蛋白杂合体筛查，以确保输血安全。通过将人B淋巴细胞与骨髓瘤细胞（JMS-3）融合，建立了四个分泌抗-Mi、抗-MUT和抗-Mur的人杂交瘤克隆。对每种单克隆抗体（MoAb）的特异性进行了表征。三种MoAb应用于自动输血前血液检测分析仪（PK7300/PK7400）进行献血者筛查。进行基因分型以确定血型糖蛋白杂合体的详细亚群。四种MoAb均为IgM抗体。抗-Mi（377T）与DXHKRDTYA、HKRDTYAAHT肽结合，抗-Mi（367T）与QTNDXHKRD肽结合（X表示T、M或K）。抗-Mur与KRDTYPAHTA肽反应。抗-MUT与KHKRDTYA反应。使用三种MoAb对总共78327名献血者进行了筛查，其中3690名（4.71%）为GP.Mur，20名（0.025%）为GP.Hut，18名（0.022%）为GP.Vw。当将Mi抗原作为常规筛查项目时，台湾献血者中Mi(a+)的频率为4.66%（67348/1444541）。Mi抗原已作为常规血液检测项目实施，结果标注在所有红细胞（RBC）单位上。
