一种用于检测内源性次氯酸的高分辨率线粒体靶向比率荧光探针。
A high-resolution mitochondria-targeting ratiometric fluorescent probe for detection of the endogenous hypochlorous acid.
作者信息
Zhou Liyi, Lu Dan-Qing, Wang Qianqian, Hu Shunqin, Wang Haifei, Sun Hongyan, Zhang Xiaobing
机构信息
College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China; Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China; Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Sep 5;166:129-134. doi: 10.1016/j.saa.2016.05.019. Epub 2016 May 16.
Hypochlorite anion, one of the biologically important reactive oxygen species, plays an essential role in diverse normal biochemical functions and abnormal pathological processes. Herein, an efficient high-resolution mitochondria-targeting ratiometric fluorescent probe for hypochlorous acid detection has been designed, synthesized and characterized. It is easily synthesized by the condensation reaction (CC) of a 2-(2-hydroxyphenyl) quinazolin-4(3H)-one fluorophore and a cyanine group (mitochondria-targeting), which made the whole molecular a large Stokes shift (210nm) and the two well-resolved emission peaks separated by 140nm. As a result, it is considered as a good candidate for high resolution hypochlorous acid imaging in live cells. The ratiometric fluorescent probe exhibited outstanding features of high sensitivity, high selectivity, rapid response time (within 50s), and excellent mitochondria-targeting ability. Moreover, the probe can also be successfully applied to imaging endogenously hypochlorous acid in the mitochondria of living cells with low cytotoxicity, and high resolution.
次氯酸根阴离子是具有重要生物学意义的活性氧物种之一，在多种正常生化功能和异常病理过程中发挥着至关重要的作用。在此，设计、合成并表征了一种用于检测次氯酸的高效高分辨率线粒体靶向比率荧光探针。它通过2-(2-羟基苯基)喹唑啉-4(3H)-酮荧光团与一个花菁基团（线粒体靶向）的缩合反应（CC）轻松合成，这使得整个分子具有较大的斯托克斯位移（210nm）以及两个分辨率良好、相隔140nm的发射峰。因此，它被认为是活细胞中高分辨率次氯酸成像的良好候选物。该比率荧光探针具有高灵敏度、高选择性、快速响应时间（50秒内）和出色的线粒体靶向能力等突出特点。此外，该探针还能成功应用于对活细胞线粒体内内源性次氯酸进行成像，具有低细胞毒性和高分辨率。
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