Jarugula Sridhar, Charlesworth Steven R, Qu Feng, Stewart Lucy R
Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA.
Department of Plant Pathology, Ohio State University, OARDC, Wooster, OH, USA.
Arch Virol. 2016 Aug;161(8):2291-7. doi: 10.1007/s00705-016-2863-2. Epub 2016 May 28.
A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis.
构建了一种用于农杆菌介导的土壤传播小麦花叶病毒（SBWMV；帚状病毒属；烟草花叶病毒科）全长感染性cDNA克隆。克隆的病毒可通过农杆菌浸润法导入本氏烟草，随后感染小麦（普通小麦）。通过在RNA2的多个位点插入序列，评估了该病毒作为基因沉默和表达载体的效用。尽管插入片段稳定，但在本氏烟草中观察到病毒诱导的光漂白现象，而在小麦中未观察到。该SBWMV感染性克隆可用于进一步研究，通过诱变来探究SBWMV的生物学特性。
