Krauzová Eva, Kračmerová Jana, Rossmeislová Lenka, Mališová Lucia, Tencerová Michaela, Koc Michal, Štich Vladimír, Šiklová Michaela
Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Second Department of Internal Medicine, University Hospital Královské Vinohrady, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
Atherosclerosis. 2016 Jul;250:151-7. doi: 10.1016/j.atherosclerosis.2016.04.021. Epub 2016 Apr 26.
Obesity represents a high risk factor for the development of atherosclerosis and is associated with a low-grade inflammation and activation of immune cells.
The aim of our study was to investigate the effect of a short-term lipid infusion on immune cells in blood and subcutaneous abdominal adipose tissue (SAAT) in obese women.
Seven-hour intravenous lipid/control infusions were performed in two groups of women (n = 15, n = 10, respectively). Before and at the end of the infusion, SAAT and blood samples were obtained and relative content and phenotype of immune cells were analyzed using flow cytometry. Analysis of immune cell markers, inflammation and angiogenesis markers was performed in SAAT by RT-PCR and in plasma by immunoassays.
Relative content of CD45+/14+ and CD45+/14+/16+ populations of monocytes was reduced in circulation by 21% (p = 0.004) and by 46% (p = 0.0002), respectively, in response to hyperlipidemia, which suggested the increased adhesion of these cells to endothelium. In line with this, the levels of sICAM and sVCAM in plasma were increased by 9.4% (p = 0.016), 11.8% (p = 0.008), respectively. In SAAT, the relative content of M2 monocyte/macrophages subpopulation CD45+/14+/206+/16+ decreased by 27% (p = 0.012) and subpopulations CD14+/CD206- and CD14/+TLR4+ cells increased (p = 0.026; p = 0.049, respectively). Intralipid infusion promoted an increase of mRNA levels in SAAT: RORC (marker of proinflammatory Th17 lymphocytes) by 43% (p = 0.048), MCP-1 (78%, p = 0.028) and VEGF (68.5%, p = 0.0001).
Acute hyperlipidemia induces a proinflammatory and proatherogenic response associated with altered relative content of immune cells in blood and SAAT in obese women.
肥胖是动脉粥样硬化发生的高风险因素，与低度炎症及免疫细胞激活相关。
我们研究的目的是调查短期脂质输注对肥胖女性血液及腹部皮下脂肪组织（SAAT）中免疫细胞的影响。
对两组女性（分别为n = 15，n = 10）进行7小时静脉脂质/对照输注。在输注前及输注结束时，采集SAAT及血液样本，使用流式细胞术分析免疫细胞的相对含量及表型。通过RT-PCR在SAAT中及通过免疫测定在血浆中进行免疫细胞标志物、炎症及血管生成标志物的分析。
高脂血症导致循环中CD45+/14+单核细胞群体及CD45+/14+/16+单核细胞群体的相对含量分别降低21%（p = 0.004）及46%（p = 0.0002），这表明这些细胞与内皮的黏附增加。与此一致，血浆中sICAM和sVCAM水平分别升高9.4%（p = 0.016）、11.8%（p = 0.008）。在SAAT中，M2单核细胞/巨噬细胞亚群CD45+/14+/206+/16+的相对含量降低27%（p = 0.012），而CD14+/CD206-及CD14/+TLR4+细胞亚群增加（分别为p = 0.026；p = 0.049）。脂质乳剂输注促使SAAT中mRNA水平升高：促炎Th17淋巴细胞标志物RORC升高43%（p = 0.048），MCP-1升高78%（p = 0.028），VEGF升高68.5%（p = 0.0001）。
急性高脂血症诱导肥胖女性血液及SAAT中免疫细胞相对含量改变，引发促炎及促动脉粥样硬化反应。
