胃癌中结直肠癌缺失基因位点的杂合性缺失及mRNA表达
Loss of heterozygosity and mRNA expression at deleted in colorectal cancer gene locus in gastric cancer.
作者信息
Wang D X, Fang D C, Luo Y H, Liu W W
机构信息
Dong-Xu Wang, Dian-Chun Fang, Yuan-Hui Luo, Wei-Wen Liu, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
出版信息
World J Gastroenterol. 1997 Sep 15;3(3):156-9. doi: 10.3748/wjg.v3.i3.156.
AIM
To assess the effects of the deleted in colorectal cancer (DCC) gene changes on the development and progression of gastric cancer.
METHODS
The loss of heterozygosity (LOH) and mRNA expression DCC gastric cancer using a PCR-based detection method.
RESULTS
LOH was found in 35.3% (18/51) of the specimens, and the LOH was more frequently detected in tumors from patients with stage III or IV cancer (50.5%) than those in stages I or II (14.3%) (P < 0.05). The occurrence of LOH was not found to correlate with the histological type, tumor size, invasion depth and lymph node metastasis of gastric cancer. The mRNA expression of the DCC gene was studied in 26 of the 51 cases, of which LOE was found in 30.8% (8/26). LOE was not significantly correlated to LOH or other clinicopathological parameters.
CONCLUSION
LOH and LOE of DCC gene are frequently encountered in gastric cancer, and the LOH of DCC gene is a late event associated with progression of gastric cancer.
目的
评估结直肠癌缺失基因（DCC）的改变对胃癌发生及进展的影响。
方法
采用基于聚合酶链反应的检测方法检测胃癌中杂合性缺失（LOH）及DCC基因的mRNA表达情况。
结果
在51份标本中有35.3%（18/51）检测到LOH，III期或IV期癌症患者肿瘤中LOH的检出率（50.5%）高于I期或II期患者（14.3%）（P<0.05）。未发现LOH的发生与胃癌的组织学类型、肿瘤大小、浸润深度及淋巴结转移相关。在51例中的26例研究了DCC基因的mRNA表达，其中30.8%（8/26）发现存在表达缺失（LOE）。LOE与LOH或其他临床病理参数无显著相关性。
结论
DCC基因的LOH和LOE在胃癌中较为常见，DCC基因的LOH是与胃癌进展相关的晚期事件。
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