通过浊点萃取分光光度法快速评估血浆和血清中的铁
Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction.
作者信息
Samarina Tatyana, Proskurnin Mikhail
机构信息
Chemistry Department, Agilent Technologies Partner Laboratory - Analytical Centre, M.V. Lomonosov Moscow State University, Moscow, 119991, Russian Federation.
出版信息
F1000Res. 2015 Aug 25;4:623. doi: 10.12688/f1000research.6716.1. eCollection 2015.
Abstract
Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5-6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of quantitation is 0.1 μg/mL, the determination range is 0.1 - 2.8 μg/mL (RSD 0.02-0.10). Copper does not interfere with the iron assessment.
摘要
提出了一种在加热至浊点时，在非离子表面活性剂的胶束相中用1-亚硝基-2-萘酚萃取铁（II）配合物后，通过简单程序对血浆和血清中的铁进行快速光度评估的方法（pH范围为4.5 - 6.3）。使用邻二氮菲的标准参考方法验证了该方法的准确性。该方法的优点是比参考方法具有更高的灵敏度：检测限为0.03μg/mL，定量限为0.1μg/mL，测定范围为0.1 - 2.8μg/mL（相对标准偏差为0.02 - 0.10）。铜不干扰铁的评估。
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