Du Jingjing, Li Qiang, Shen Linyuan, Lei Huaigang, Luo Jia, Liu Yihui, Zhang Peiwen, Pu Qiang, Zhang Yi, Shuai Surong, Li Xuewei, Zhang Shunhua, Zhu Li
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Sichuan Province General Station of Animal Husbandry, Chengdu 611130, China.
Biomed Res Int. 2016;2016:5276271. doi: 10.1155/2016/5276271. Epub 2016 Apr 28.
MicroRNAs are a class of 18-22-nucleotide noncoding RNAs that posttranscriptionally regulate gene expression and have been shown to play an important role during myoblast differentiation. In this study, we found that the expression of miR-145a-5p was gradually increased during C2C12 myoblast differentiation, and miR-145a-5p inhibitors or mimics significantly suppressed or promoted the relative expression of specific myogenesis related marker genes. Moreover, overexpression or inhibition of miR-145a-5p enhanced or repressed the expression of some special genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicated that miR-145a-5p might be considered as a new myogenic differentiation-associated microRNA that can promote C2C12 myoblast differentiation by enhancing genes related to myoblasts differentiation.
微小RNA是一类18 - 22个核苷酸的非编码RNA，它们在转录后调节基因表达，并且已被证明在成肌细胞分化过程中发挥重要作用。在本研究中，我们发现miR - 145a - 5p在C2C12成肌细胞分化过程中表达逐渐增加，并且miR - 145a - 5p抑制剂或模拟物显著抑制或促进特定成肌相关标记基因的相对表达。此外，在C2C12成肌细胞分化过程中，miR - 145a - 5p的过表达或抑制增强或抑制了参与内源性Wnt信号通路的一些特殊基因的表达，包括Wnt5a、LRP5、Axin2和β - 连环蛋白。这些结果表明，miR - 145a - 5p可能被视为一种新的与成肌分化相关的微小RNA，它可以通过增强与成肌细胞分化相关的基因来促进C2C12成肌细胞分化。
