miR-143-3p对C2C12成肌细胞分化的影响。
Effect of miR-143-3p on C2C12 myoblast differentiation.
作者信息
Du Jingjing, Zhang Yi, Shen Linyuan, Luo Jia, Lei Huaigang, Zhang Peiwen, Pu Qiang, Liu Yihui, Shuai Surong, Li Qiang, Li Xuewi, Zhang Shunhua, Zhu Li
机构信息
a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
b Department of Animal Science , Xichang College , Xichang , China.
出版信息
Biosci Biotechnol Biochem. 2016;80(4):706-11. doi: 10.1080/09168451.2015.1123604. Epub 2016 Feb 8.
MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3' untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.
微小RNA是一类由18 - 22个核苷酸组成的非编码RNA，它们通过与mRNA的3'非翻译区结合来调节基因表达。大量微小RNA参与成肌细胞分化的调控，其中许多仍未被发现。在本研究中，我们发现miR - 143 - 3p在C2C12成肌细胞分化过程中上调，miR - 143 - 3p的过表达显著抑制MyoD、MyoG、myf5和MyHC基因的相对表达水平，尤其是在分化后期。此外，miR - 143 - 3p在C2C12成肌细胞分化过程中抑制内源性Wnt信号通路相关基因的表达，包括Wnt5a、LRP5、Axin2和β - 连环蛋白。这些结果表明，miR - 143 - 3p是一种新的与肌源性分化相关的微小RNA，它可以抑制C2C12成肌细胞分化，尤其是在分化后期。
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