Du Jingjing, Zhang Yi, Shen Linyuan, Luo Jia, Lei Huaigang, Zhang Peiwen, Pu Qiang, Liu Yihui, Shuai Surong, Li Qiang, Li Xuewi, Zhang Shunhua, Zhu Li
a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
b Department of Animal Science , Xichang College , Xichang , China.
Biosci Biotechnol Biochem. 2016;80(4):706-11. doi: 10.1080/09168451.2015.1123604. Epub 2016 Feb 8.
MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3' untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.
微小RNA是一类由18 - 22个核苷酸组成的非编码RNA，它们通过与mRNA的3'非翻译区结合来调节基因表达。大量微小RNA参与成肌细胞分化的调控，其中许多仍未被发现。在本研究中，我们发现miR - 143 - 3p在C2C12成肌细胞分化过程中上调，miR - 143 - 3p的过表达显著抑制MyoD、MyoG、myf5和MyHC基因的相对表达水平，尤其是在分化后期。此外，miR - 143 - 3p在C2C12成肌细胞分化过程中抑制内源性Wnt信号通路相关基因的表达，包括Wnt5a、LRP5、Axin2和β - 连环蛋白。这些结果表明，miR - 143 - 3p是一种新的与肌源性分化相关的微小RNA，它可以抑制C2C12成肌细胞分化，尤其是在分化后期。
