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血清淀粉样蛋白 P 成分对 RAW264.7 小鼠巨噬细胞基因表达的影响。

The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages.

机构信息

Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.

出版信息

Biomed Res Int. 2016;2016:9380290. doi: 10.1155/2016/9380290. Epub 2016 Apr 28.

DOI:10.1155/2016/9380290
PMID:27239478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4864538/
Abstract

Serum amyloid P-component (SAP) contributes to host defense and prevents fibrosis. Macrophages are the most abundant inflammatory cell type in atherosclerotic plaques. In the present study, using (3)H-cholesterol-labeled counting radioactivity assay, we demonstrated that the apoAI-mediated cholesterol efflux in RAW264.7 macrophages was increased by SAP treatment in a time- and dose-dependent manner. We analyzed global gene expression changes upon SAP treatment using RNA sequencing. As a result, a total of 175 differentially expressed genes were identified, of which 134 genes were downregulated and 41 genes were upregulated in SAP treated cells compared to control cells. Quantitative RT-PCR analysis confirmed decreased expression of 5 genes and an increase in expression of 1 gene upon SAP treatment. Gene ontology analysis showed that genes involved in response to stimulus were significantly enriched in differentially expressed genes. Beyond protein-coding genes, we also identified 8 differentially expressed long noncoding RNAs. Our study may provide new insights into mechanisms underlying the functional role of SAP in macrophages.

摘要

血清淀粉样蛋白 P 成分（SAP）有助于宿主防御并防止纤维化。巨噬细胞是动脉粥样硬化斑块中最丰富的炎症细胞类型。在本研究中，我们使用（3）H-胆固醇标记的计数放射性测定法，证明 SAP 处理以时间和剂量依赖的方式增加了 RAW264.7 巨噬细胞中 apoAI 介导的胆固醇流出。我们使用 RNA 测序分析了 SAP 处理后的全基因表达变化。结果，共鉴定出 175 个差异表达基因，其中 SAP 处理细胞与对照细胞相比，有 134 个基因下调，41 个基因上调。定量 RT-PCR 分析证实，SAP 处理后 5 个基因的表达下调，1 个基因的表达上调。基因本体分析表明，参与对刺激的反应的基因在差异表达基因中显著富集。除了蛋白质编码基因外，我们还鉴定了 8 个差异表达的长非编码 RNA。我们的研究可能为 SAP 在巨噬细胞中的功能作用的机制提供新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/d1a6583451ae/BMRI2016-9380290.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/0fa77f6a5e99/BMRI2016-9380290.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/7e5239d0e929/BMRI2016-9380290.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/f3366ebed1dd/BMRI2016-9380290.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/d1a6583451ae/BMRI2016-9380290.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/0fa77f6a5e99/BMRI2016-9380290.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/7e5239d0e929/BMRI2016-9380290.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/f3366ebed1dd/BMRI2016-9380290.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/368b/4864538/d1a6583451ae/BMRI2016-9380290.004.jpg

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