Park Yun Ji, Arasu Mariadhas Valan, Al-Dhabi Naif Abdullah, Lim Soon Sung, Kim Yeon Bok, Lee Sang Won, Park Sang Un
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Molecules. 2016 May 27;21(6):691. doi: 10.3390/molecules21060691.
Valeriana fauriei (V. fauriei), which emits a characteristic and unpleasant odor, is important in traditional medicine. In this study, the expression of terpenoid biosynthetic genes was investigated in different organs that were also screened for volatile compounds including valerenic acid and its derivatives. Specific expression patterns from different parts of V. fauriei were observed using quantitative real-time PCR (qRT-PCR). The highest transcript levels of biosynthetic genes involved in mevalonic acid (MVA) and methylerythritol phosphate (MEP) production were found in the stem. Although the amounts of volatile compounds were varied by organ, most of the volatile terpenoids were accumulated in the root. Gas chromatography mass spectrometry (GC-MS) analysis identified 128 volatile compounds, which represented 65.33% to 95.66% of total volatiles. Certain compounds were only found in specific organs. For example, isovalerenic acid and valerenic acid and its derivatives were restricted to the root. Organs with high transcript levels did not necessarily have high levels of the corresponding chemical constituents. According to these results, we hypothesize that translocation may occur between different organs in V. fauriei.
朝鲜缬草（Valeriana fauriei）会散发一种独特且难闻的气味，在传统医学中具有重要地位。在本研究中，对朝鲜缬草不同器官中萜类生物合成基因的表达进行了研究，同时还对包括缬草烯酸及其衍生物在内的挥发性化合物进行了筛选。使用定量实时PCR（qRT-PCR）观察了朝鲜缬草不同部位的特定表达模式。参与甲羟戊酸（MVA）和甲基赤藓糖醇磷酸（MEP）生成的生物合成基因的转录水平在茎中最高。尽管挥发性化合物的含量因器官而异，但大多数挥发性萜类化合物都积累在根部。气相色谱-质谱联用（GC-MS）分析鉴定出128种挥发性化合物，占总挥发物的65.33%至95.66%。某些化合物仅在特定器官中被发现。例如，异缬草烯酸、缬草烯酸及其衍生物仅限于根部。转录水平高的器官不一定具有高水平的相应化学成分。根据这些结果，我们推测朝鲜缬草不同器官之间可能发生转运。
