Nadeem A, Maryam J
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
Trop Anim Health Prod. 2016 Aug;48(6):1261-8. doi: 10.1007/s11250-016-1086-6. Epub 2016 May 30.
Milk yield and quality has been a major selection criterion for genetic improvement in livestock species. Role of Prolactin gene in determining milk quality in terms of protein profile, lactose, lipids and other imperative macromolecules is very important. In this context, genetic profiling of Prolactin gene in riverine buffalo of Pakistan was performed and potential genetic markers were identified illustrating worth of this gene in marker-assisted selection of superior dairy buffaloes. Series of wet and dry lab experimentation was performed starting with genomic DNA isolation from true to breed representatives of indigenous river buffalo (Nili-Ravi). After amplification of coding regions of Prolactin gene, products were eluted and sequenced by Sanger's chain termination method and aligned to get variations in genomic region. A total of 15 novel variations were identified and analyzed statistically for their significance at population level, haplotypes were constructed, and association was estimated. Phylogenetic analysis was performed to evaluate the rate of evolution for Prolactin gene in various mammalian species. Lastly, biological networking for this molecule was predicted to get the bigger pictorial of its functional machinery. Pathway analysis was performed to find its physiological mode of action in milk synthesis. This is a first report toward complete genetic screening of Prolactin gene in Pakistani buffaloes. Results of this study not only provide an insight for potential role of Prolactin gene in milk-producing abilities of buffalo but also suggest new directions for exploration of more genes that may have promising role to enhance future milk production capabilities of river buffalo breeds of Asian region through marker-assisted selection.
产奶量和奶品质一直是家畜品种遗传改良的主要选择标准。催乳素基因在决定奶的蛋白质组成、乳糖、脂质及其他重要大分子方面的奶品质中起着非常重要的作用。在此背景下，对巴基斯坦河流型水牛的催乳素基因进行了基因分型，并鉴定出潜在的遗传标记，说明了该基因在优质奶牛水牛标记辅助选择中的价值。开展了一系列湿实验室和干实验室实验，首先从纯种本土河流型水牛（尼里-拉菲）的代表中分离基因组DNA。在扩增催乳素基因的编码区后，洗脱产物并采用桑格链终止法进行测序，然后进行比对以获得基因组区域的变异。共鉴定出15个新变异，并在群体水平上对其显著性进行了统计分析，构建了单倍型，并估计了关联性。进行了系统发育分析，以评估催乳素基因在各种哺乳动物物种中的进化速率。最后，预测了该分子的生物网络，以更全面地了解其功能机制。进行了通路分析，以发现其在乳汁合成中的生理作用模式。这是关于巴基斯坦水牛催乳素基因完整基因筛选的第一份报告。本研究结果不仅为催乳素基因在水牛产奶能力中的潜在作用提供了见解，也为探索更多基因指明了新方向，这些基因可能通过标记辅助选择在提高亚洲地区河流型水牛品种未来产奶能力方面发挥重要作用。
