Liu Jun, Li Zhihong, Wei Yanru, Wang Wenjia, Wang Bing, Liang Hongli, Gao Yuxi
Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
Protein Sci. 2016 Aug;25(8):1385-9. doi: 10.1002/pro.2957. Epub 2016 Jun 16.
By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.
通过对由致密单分散体系的小角X射线散射(SAXS)理论数据得出的距离分布函数(DDF)进行模拟,我们发现了球形对称(或近似球形对称)均匀颗粒的表观尺寸与溶液浓度之间的定量数学关联。对人血红蛋白和马肌红蛋白蛋白质溶液进行的SAXS实验验证了这种关联。这给出了一种从SAXS DDF确定致密单分散体系中球形对称(或近似球形对称)颗粒尺寸的新方法,特别是对于具有干涉效应的蛋白质溶液。