Degnan B M, Hawkins C J, Lavin M F, McCaffrey E J, Parry D L, Watters D J
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
J Med Chem. 1989 Jun;32(6):1354-9. doi: 10.1021/jm00126a035.
The isolation and structures of two new cyclic hexapeptides and two new macrocyclic ethers from the aplousobranch ascidian Lissoclinum bistratum are described. Their structures were determined by two-dimensional NMR techniques. The hexapeptides, named bistratamide A and bistratamide B, differ only by the presence or absence of one double bond. They were tested for cytotoxicity toward human fibroblast and tumor cell lines and displayed similar toxicities to the octapeptides called patellamides from Lissoclinum patella. The peptides are found within the obligate algal symbiont Prochloron but clearly differ from peptides isolated from the same Prochloron of L. patella. The macrocyclic ethers isolated from L. bistratum are exceedingly potent in cytotoxicity. They have been named bistratenes A and B, and structures for these compounds are proposed.
本文描述了从软鳃海鞘双纹 Lissoclinum bistratum 中分离得到的两种新型环六肽和两种新型大环醚及其结构。它们的结构通过二维核磁共振技术确定。这两种环六肽分别命名为双纹酰胺 A 和双纹酰胺 B，仅因一个双键的有无而有所不同。它们针对人成纤维细胞和肿瘤细胞系进行了细胞毒性测试，结果显示与从 L. patella 中分离得到的八肽类海鞘酰胺具有相似的毒性。这些肽存在于专性藻类共生体原绿藻中，但与从 L. patella 的同一原绿藻中分离得到的肽明显不同。从双纹 Lissoclinum bistratum 中分离得到的大环醚具有极强的细胞毒性。它们被命名为双纹烯 A 和双纹烯 B，并提出了这些化合物的结构。
