Zhu X X, Oliver J H, Vogel G N
J Med Entomol. 1989 May;26(3):217-21. doi: 10.1093/jmedent/26.3.217.
Injected [3H]precocene-II spread from the hemocoel of Ornithodoros parkeri (Cooley) ticks to gut, salivary glands, synganglion, ovary, and the remainder of the body (carcass). Analyses at 2, 24, and 48 h after injection of precocene revealed that radioactivity remained at low levels in the synganglia and ovaries of fed-mated and fed-virgin females. Radioactivity increased gradually during 48 h after injection in salivary glands of fed-mated and fed-virgin ticks. It also increased gradually in the gut of fed virgins but not in fed-mated females. The carcasses contained the majority of total radioactivity at 2, 24, and 48 h after injection. Most of the radioactivity was found in ether-soluble compounds and only minimal amounts in water-soluble metabolites. Analysis of tick excreta and washings indicate that female O. parkeri excrete injected precocene very slowly; only 6% of total radioactivity was recovered in these samples during the 48 h after injection. Thin-layer chromatographic analyses of ether extracts of salivary glands and carcasses from fed-mated ticks 24 h after injection showed that the majority of the injected [3H]precocene-II was present in unchanged form.
注射的[³H]早熟素II从帕克钝缘蜱（库利）的血腔扩散到肠道、唾液腺、交感神经节、卵巢和身体其他部位（躯体）。在注射早熟素后2小时、24小时和48小时进行的分析表明，放射性在已进食交配和已进食未交配雌蜱的交感神经节和卵巢中保持在低水平。在已进食交配和已进食未交配蜱的唾液腺中，放射性在注射后48小时内逐渐增加。在已进食未交配雌蜱的肠道中放射性也逐渐增加，但在已进食交配雌蜱的肠道中则没有。在注射后2小时、24小时和48小时，躯体含有大部分总放射性。大部分放射性存在于醚溶性化合物中，而水溶性代谢物中的含量极少。对蜱排泄物和冲洗液的分析表明，帕克钝缘蜱雌蜱排泄注射的早熟素非常缓慢；在注射后48小时内，这些样品中仅回收了6%的总放射性。对注射后24小时的已进食交配蜱的唾液腺和躯体的醚提取物进行的薄层色谱分析表明，注射的[³H]早熟素II大部分以未改变的形式存在。
