Disanto Maria Giulia, Ambrosio Maria Raffaella, Rocca Bruno Jim, Ibrahim Hazem A H, Leoncini Lorenzo, Naresh Kikkeri N
From the Department of Histopathology, Imperial College Healthcare NHS Trust & Imperial College, London, United Kingdom.
Department of Medical Biotechnologies, Section of Pathology, University of Siena, Siena, Italy.
Am J Clin Pathol. 2016 May;145(5):687-95. doi: 10.1093/ajcp/aqw060.
Establish and validate optimal minimal immunohistochemistry panels for usage in a staged algorithmic manner for precise diagnosis of B-cell lymphomas in countries with limited resources. Suggest short panels of immunostains to be used in referring units that refer suspected lymphomas to specialist diagnostic centers in resourceful countries.
Significant proportion of six B-cell lymphomas has characteristic morphology requiring a short panel of confirmatory immunostains. The rest would go through five different algorithms.
812 cases in which a B-cell lymphoma or an HIV-associated lymphoma was suspected on morphological grounds were evaluated. This led to arriving at a specific diagnosis of 799 B-cell lymphomas. A correct diagnosis was achievable in 69% cases with the application of three to five antibodies; others required additional work-up.
The panels/algorithms assist pathologists in practicing lymphoma diagnostics in countries with limited resources and in making lymphoma referrals to specialist centers.
建立并验证最佳的最小免疫组织化学检测组合，以便在资源有限的国家以分阶段算法方式用于精确诊断B细胞淋巴瘤。建议在将疑似淋巴瘤转诊至资源丰富国家的专科诊断中心的转诊单位中使用简短的免疫染色检测组合。
六种B细胞淋巴瘤中有很大一部分具有特征性形态，需要一组简短的确认性免疫染色。其余的将通过五种不同的算法进行分析。
对812例因形态学原因怀疑为B细胞淋巴瘤或HIV相关淋巴瘤的病例进行了评估。这导致对799例B细胞淋巴瘤做出了明确诊断。应用三到五种抗体可在69%的病例中实现正确诊断；其他病例则需要进一步检查。
这些检测组合/算法有助于资源有限国家的病理学家进行淋巴瘤诊断，并将淋巴瘤转诊至专科中心。
