Rana Manjit K, Rana Amrit Pal S, Jain Aklank, Pathak Akhilesh, Khera Utkarshni, Sharma Uttam, Jindal Akriti, Singh Karuna
Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Bathinda, IND.
General Surgery, All India Institute of Medical Sciences, Bathinda, IND.
Cureus. 2022 Jun 8;14(6):e25773. doi: 10.7759/cureus.25773. eCollection 2022 Jun.
Immunohistochemistry (IHC) is a necessary ancillary technique in surgical pathology laboratories, particularly for oncology tissue specimens. Automation in the IHC technique has an advantage over manual methods in terms of quality, except for the cost of the equipment. Thus, the manual method of IHC staining is the preferred method of choice in countries with limited resources. However, standardization of all steps in the preanalytic phase is critical to obtain reliable immunohistochemistry test results. The current audit was conducted to describe the preanalytic factors affecting manual IHC methods. The most important preanalytic factors were fixative, the composition of dehydrate, pH, drying of sections, and heat-mediated antigen retrieval method (HMAR). The domestic pressure cooker method was found to be the best for HMAR.
免疫组织化学（IHC）是外科病理实验室中一项必要的辅助技术，尤其适用于肿瘤组织标本。免疫组织化学技术的自动化在质量方面优于手工方法，只是设备成本较高。因此，在资源有限的国家，免疫组织化学染色的手工方法是首选方法。然而，分析前阶段所有步骤的标准化对于获得可靠的免疫组织化学检测结果至关重要。本次审核旨在描述影响手工免疫组织化学方法的分析前因素。最重要的分析前因素是固定剂、脱水剂成分、pH值、切片干燥以及热介导抗原修复方法（HMAR）。结果发现，家用高压锅法是热介导抗原修复的最佳方法。
