Uchibayashi T, Egawa M, Asari T, Nakajima K, Hisazumi H, Endo Y, Tanaka M, Sasaki T
Nihon Hinyokika Gakkai Zasshi. 1989 Jan;80(1):17-21. doi: 10.5980/jpnjurol1989.80.17.
The chorioallantoic membrane of chick embryo was used to examine the responses of tumor cell lines and surgically resected specimens to anticancer agents in combination with or without hyperthermia. B16-F10 melanoma, KK-47 cell line derived from a transitional cell carcinoma of the bladder and surgical specimens from urological malignancies were transplanted onto the chorioallantoic membrane of chick embryo 10 days after fertilization. The effects of intravascularly injected cyclophosphamide, cisplatin, adriamycin, mitomycin C, vincristine and vinblastine, in combination with or without hyperthermia, on the growth of the grafts were investigated. A 15-minute 42.5 degrees C hyperthermia exhibited most favorable anticancer effects in B16-F10 grafts (P less than 0.01) of all heating conditions tested. A combined use of hyperthermia and cisplatin, cyclophosphamide or adriamycin demonstrated a significantly high tumor regression rate in B16-F10 grafts (P less than 0.01). In KK-47 grafts, a single use of cyclophosphamide and a combined use of hyperthermia and cyclophosphamide exhibited a highly significant antitumor regression (P less than 0.05). Surgical specimens from 14 urological malignancies were subjected to this assay. Growth which was adequate for sensitivity test was obtained in 11 of the 14 malignancies. The results obtained suggest that this assay system may be a useful chemohyperthermia sensitivity test.
鸡胚绒毛尿囊膜被用于检测肿瘤细胞系和手术切除标本在联合或不联合热疗情况下对抗癌药物的反应。将B16-F10黑色素瘤、源自膀胱移行细胞癌的KK-47细胞系以及泌尿生殖系统恶性肿瘤的手术标本，在受精后10天移植到鸡胚绒毛尿囊膜上。研究了血管内注射环磷酰胺、顺铂、阿霉素、丝裂霉素C、长春新碱和长春花碱，联合或不联合热疗，对移植物生长的影响。在所有测试的加热条件下，15分钟42.5摄氏度的热疗在B16-F10移植物中表现出最有利的抗癌效果（P<0.01）。热疗与顺铂、环磷酰胺或阿霉素联合使用，在B16-F10移植物中显示出显著高的肿瘤消退率（P<0.01）。在KK-47移植物中，单独使用环磷酰胺以及热疗与环磷酰胺联合使用均表现出高度显著的抗肿瘤消退作用（P<0.05）。对14例泌尿生殖系统恶性肿瘤的手术标本进行了该检测。14例恶性肿瘤中有11例获得了足以进行敏感性测试的生长情况。所得结果表明，该检测系统可能是一种有用的化学热疗敏感性测试。
