Uchida H, Sasaki T, Tanaka M, Endo Y, Nitta K, Nishikawa K, Chuman H, Fukuma H, Matsumoto K
Jpn J Cancer Res. 1987 Jul;78(7):729-36.
The chorioallantoic membrane of chick embryo was used to examine the chemosensitivity of the murine tumors, B16-F1 melanoma, B16-F10 melanoma, Meth-A fibrosarcoma, and Ehrlich carcinoma. The tumors were grown on the membrane, and the effects of 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride, cyclophosphamide, dacarbazine, 5-fluorouracil, methotrexate, adriamycin, mitomycin C, vincristine, and cisplatin on the growth of the tumors were tested by iv injection into a vein of the chorioallantoic membrane or injection into the yolk sac. The use of chick embryo limits the time for drug exposure to 3 or 4 days, but Ehrlich carcinoma and Meth-A fibrosarcoma needed longer for the test; the use of irradiation from a cobalt source overcame the problem by increasing the growth rate of the grafts of these two tumors. There appeared to be a good correlation between the effects of the drugs on B16 melanomas grown in the eggs and in the original animals. Many compounds, including pro-drugs such as cyclophosphamide and dacarbazine, could be assayed in this way.
利用鸡胚的绒毛尿囊膜检测小鼠肿瘤（B16 - F1黑色素瘤、B16 - F10黑色素瘤、Meth - A纤维肉瘤和艾氏癌）的化学敏感性。将肿瘤接种于该膜上，通过向绒毛尿囊膜静脉内注射或向卵黄囊内注射，测试1 -（4 - 氨基 - 2 - 甲基 - 5 - 嘧啶基）甲基 - 3 -（2 - 氯乙基）- 3 - 亚硝基脲盐酸盐、环磷酰胺、达卡巴嗪、5 - 氟尿嘧啶、甲氨蝶呤、阿霉素、丝裂霉素C、长春新碱和顺铂对肿瘤生长的影响。使用鸡胚将药物暴露时间限制在3或4天，但艾氏癌和Meth - A纤维肉瘤的检测需要更长时间；使用钴源照射通过提高这两种肿瘤移植瘤的生长速率解决了这个问题。药物对在卵内生长的B16黑色素瘤和原动物体内B16黑色素瘤的作用之间似乎存在良好的相关性。许多化合物，包括环磷酰胺和达卡巴嗪等前体药物，都可以用这种方法进行检测。
