Uchibayashi T, Egawa M, Nakajima K, Hisazumi H, Tanaka M, Endo Y, Sasaki T
Department of Urology, School of Medicine, Kanazawa University, Japan.
Urol Res. 1992;20(3):237-9. doi: 10.1007/BF00299724.
The chorioallantoic membrane of chick embryos was used to examine the responses of three tumour cell lines to anticancer agents, alone and in combination with hyperthermia. Fifteen minutes of hyperthermia at 42.5 degrees C produced the most favourable anticancer effect in the B16-F10 grafts. The use of Adriamycin (ADM) alone and the combined use of hyperthermia and either cisplatin (CDDP), cyclophosphamide (CY) or ADM resulted in a significantly higher rate of tumour regression in the B16-F10 grafts from a murine melanoma. In the KK-47 grafts derived from a transitional cell carcinoma of the bladder, the use of CY alone and the combination of CY and hyperthermia produced a significant tumour regression rate. In the T24 grafts neither the use of CY or CDDP alone, nor the combination of these drugs with hyperthermia demonstrated any significant effect. This method of screening anticancer agents was found to be rapid, simple to perform and inexpensive.
利用鸡胚的绒毛尿囊膜来检测三种肿瘤细胞系对抗癌药物的反应，以及单独使用和与热疗联合使用时的反应。在42.5摄氏度下进行15分钟的热疗对B16-F10移植瘤产生了最有利的抗癌效果。单独使用阿霉素（ADM）以及热疗与顺铂（CDDP）、环磷酰胺（CY）或ADM联合使用，均使源自小鼠黑色素瘤的B16-F10移植瘤的肿瘤消退率显著提高。在源自膀胱移行细胞癌的KK-47移植瘤中，单独使用CY以及CY与热疗联合使用均产生了显著的肿瘤消退率。在T24移植瘤中，单独使用CY或CDDP以及这些药物与热疗联合使用均未显示出任何显著效果。发现这种筛选抗癌药物的方法快速、操作简单且成本低廉。
