State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.
Appl Microbiol Biotechnol. 2016 Jul;100(14):6183-6192. doi: 10.1007/s00253-016-7650-1. Epub 2016 Jun 2.
Agrobacterium is a genus of gram-negative bacteria that can produce several typical exopolysaccharides with commercial uses in the food and pharmaceutical fields. In particular, succinoglycan and curdlan, due to their good quality in high yield, have been employed on an industrial scale comparatively early. Exopolysaccharide biosynthesis is a multiple-step process controlled by different functional genes, and various environmental factors cause changes in exopolysaccharide biosynthesis through regulatory mechanisms. In this mini-review, we focus on the genetic control and regulatory mechanisms of succinoglycan and curdlan produced by Agrobacterium. Some key functional genes and regulatory mechanisms for exopolysaccharide biosynthesis are described, possessing a high potential for application in metabolic engineering to modify exopolysaccharide production and physicochemical properties. This review may contribute to the understanding of exopolysaccharide biosynthesis and exopolysaccharide modification by metabolic engineering methods in Agrobacterium.
农杆菌是革兰氏阴性细菌的一个属，能产生几种具有商业用途的典型胞外多糖，用于食品和制药领域。特别是由于产量高、质量好，黄原胶和结冷胶很早就被工业化应用。胞外多糖生物合成是一个由不同功能基因控制的多步骤过程，各种环境因素通过调节机制引起胞外多糖生物合成的变化。在这篇综述中，我们重点关注农杆菌产生的黄原胶和结冷胶的遗传控制和调控机制。描述了一些关键的功能基因和胞外多糖生物合成的调控机制，在代谢工程中具有修饰胞外多糖生产和理化性质的高应用潜力。这篇综述可能有助于理解农杆菌中胞外多糖生物合成和代谢工程方法修饰胞外多糖。
